不同品种鹰嘴豆中α-低聚半乳糖与蔗糖的含量分析  

Determination of the Contents of α-Galactooligosaccharides and Sucrose in Different Chickpea (Cicer arietimun L.) Seeds

作　　者：向小丽[1] 杨立怡[1,2] 华双[1] 李伟[1] 孙怡[1] 麻浩[3] 张巨松[4] 曾晓雄[1] 

机构地区：[1]南京农业大学食品科技学院,南京210095 [2]Division of Food Systems and Bioengineering,College of Agriculture,Food and Natural Resources,University of Missouri-Columbia,Columbia 65211,Missouri,USA [3]南京农业大学作物遗传与种质创新国家重点实验室/农业部国家大豆改良中心,南京210095 [4]新疆农业大学农学院,乌鲁木齐830052

出　　处：《中国农业科学》2008年第9期2762-2768,共7页Scientia Agricultura Sinica

基　　金：南京农业大学高层次人才引进基金(804066)

摘　　要：【目的】分析不同品种鹰嘴豆中α-低聚半乳糖(α-GOS)与蔗糖的含量差异,以期获得α-GOS含量较高而蔗糖含量相对低的鹰嘴豆品种,为中国鹰嘴豆资源的有效利用提供理论依据。【方法】采用高效液相色谱法分析比较不同品种鹰嘴豆中的α-GOS与蔗糖的含量,利用层析技术从鹰嘴豆中分离纯化一种含量较高的未知三糖,并采用1H-NMR和ESI-TOF-MS表征了未知三糖的结构(鹰嘴豆糖醇)。【结果】在所研究的19种鹰嘴豆品种中,鹰嘴豆糖醇占总的α-GOS的比例均是最高的,约占50%;其次是水苏糖,约占35%。统计分析表明,不同品种的鹰嘴豆中蔗糖的含量差异显著,含量在1.80%～5.22%。不同品种之间α-GOS的含量也有较大差异,总α-GOS的含量在6.35%～8.68%,其中棉子糖含量0.46%～0.92%,鹰嘴豆糖醇含量3.04%～5.06%,水苏糖含量1.64%～3.09%,毛蕊花糖含量0.27%～0.70%。【结论】在19个品种的鹰嘴豆中,品种‘171’含有最高含量的α-GOS和较低含量的蔗糖,‘171’是生产α-GOS的理想原料。[Objective] The contents of α-galactooligosaccharides （α-GOS） and sucrose in different cultivars of chickpea seeds were determined with the aim of selecting those with high amounts of α-GOS and low contents of sucrose for use as ingredients in functional foods. [Method] The contents of α-GOS and sucrose in 19 kinds of chickpea seeds were determined by high performance liquid chromatography. An unknown trisaccharide, which was the main sugar in chickpea seeds, was isolated and purified by column chromatography, and identified as ciceritol by means of 1^H-NMR and ESI-TOF-MS. [Result] Ciceritol, the main sugar in all the chickpea samples, accounted for about 50% of the total α-GOS, and stachyose accounted for about 35%. Statistic analysis showed that there were significant variations in the levels of sucrose between species, ranging from 1.80%-5.22%. Substantial differences in α-GOS contents were also detected, especially in the verbascose content which could only be detected in 7 samples. The contents of raffinose, ciceritol, stachyose and verbascose ranged from 0.46%-0.92%, 3.04%-5.06%, 1.64%-3.09%, and 0.27%-0.70%, respectively. [Conclusion] With the highest amount of α-GOS and a low amount of sucrose, the variety 171 chickpea seeds was the best choice for obtaining α-GOS as prebiofics in functional foods.

关 键 词：鹰嘴豆 鹰嘴豆糖醇 蔗糖 α-低聚半乳糖 高效液相色谱法 1H-NMR ESI-TOF-MS 

分 类 号：S529[农业科学—作物学]