小麦内生多黏类芽孢杆菌hu-4产β-1,3-葡聚糖酶发酵工艺研究  

Study on β-1,3-Glucanase Fermentation Technology Produced by Wheat Endophytic Paenibacillus Polymyxa hu-4

作　　者：刘安邦[1] 王刚[2] 陈国参[1] 周伏忠[1] 宁萌[1] 

机构地区：[1]河南省微生物工程重点实验室 [2]河南大学生命科学学院,河南开封475001

出　　处：《河南科学》2008年第9期1050-1053,共4页Henan Science

基　　金：河南省教育厅自然科学基金(200510475036);河南大学重点基金项目(04ZDZR005)

摘　　要：对产β-1,3-葡聚糖酶小麦内生多黏类芽孢杆菌(Paenibacillus Polymyxa)hu-4的产酶工艺进行了研究,通过正交实验建立和优化了hu-4菌株的发酵产酶培养基,确立了最佳发酵工艺.结果表明,以蛋白胨1.8%,酵母膏0.6%,玉米浆1.2%,酵母葡聚糖0.5%,矿质元素混合溶液0.5%为其最佳发酵培养基构成,pH值7.0;在温度32℃,转速200r/min,接种量2%的工艺条件下,发酵周期为36～38h,产酶效果较好.In this paper, the β-1, 3-glucanase produced by wheat endophytic Paenibacillus polymyxa hu-4 were studyed. Optimal medium and fermentation technology were established by orthogonal design. The main composition of the medium was as follows：pH 7.0, 1.8 % peptone,0.6 % yeast extract, 1.2 % corn pulp, 0.5 % yeast glucan and 0.5 % mineral elements mixed solution. 32℃, 200 r/min and 2 % inoculum were the best fermentation conditions. The fermentation period is 36-8 h.

关 键 词：小麦内生多黏类芽孢杆菌 发酵工艺 葡聚糖酶 酵工艺 

分 类 号：S512.1[农业科学—作物学]