人乳铁蛋白在转基因马铃薯块茎中的表达  

Expression of Human Lactoferrin in Transgenic Potato Tubers

作　　者：宋东光[1] 陈小英[1] 张辉松[1] 李筠[1] 张小凤[1] 王惠珍[1] 

机构地区：[1]佛山科学技术学院园艺系,广东佛山528231

出　　处：《热带亚热带植物学报》2008年第5期430-434,共5页Journal of Tropical and Subtropical Botany

基　　金：广东省科技计划项目(2006B20101006);佛山市专项发展基金(03020121)资助

摘　　要：人乳铁蛋白(human lactoferrin,hLF)是人体非特异性免疫系统的重要成员之一,具有抗细菌、真菌和抗病毒活性及其他多种功能。报道将hLF基因的cDNA与马铃薯(Solanum tuberosum L.)块茎专一性表达patatin基因启动子融合后通过农杆菌介导导入马铃薯,PCR检测证实获得了多个转基因株系,RT-PCR阳性结果说明hLF mRNA在马铃薯植株中得到了表达。同时,经过ELISA及Western blot检测证实,转基因马铃薯表达了hLF并具有人乳铁蛋白的活性。Human lactoferrin （hLF） is a member of non-specific immune system, it plays many roles, such as resistances against bacteria, fungi and viruses, etc. In this paper, hLF cDNA directed by the tuber specific promoter of class Ⅰ patatin gene of potato （Solanum tuberosum L.） were transferred into potato mediated by Agrobacterium tumefiens transformation, hLF transgenic potato plants were confirmed by PCR detection, further RT-PCR showed that hLF RNAs were expressed in transgenic plants, hLF activities were detected by ELISA and Western blot analysis in hLF transgenic potato tubers.

关 键 词：乳铁蛋白 致转基因 马铃薯 块茎 表达 

分 类 号：S532[农业科学—作物学] Q943.2[生物学—植物学]