转trxS基因大麦发芽种子水解酶活性的变化(英文)  

Change of Hydrolase Activity in Germinating Seeds of trxS Transgenic Barley

作　　者：卫丽[1] 孔维威[2] 尹钧[1] 

机构地区：[1]河南农业大学,郑州450002 [2]南京农业大学生命科学院,南京210095

出　　处：《生物工程学报》2008年第9期1526-1530,共5页Chinese Journal of Biotechnology

摘　　要：利用转基因技术是改良大麦品种品质的有效途径。研究了转trxS基因对大麦种子发芽过程中水解酶活性的影响,结果表明转基因种子中α-淀粉酶、自由态β-淀粉酶和极限糊精酶的活性比未转基因种子高;转基因种子醇溶蛋白和谷蛋白中巯基的含量提高,说明该基因能够表达,为大麦育种和品质改良提供新的途径。Genetic modification of barley variety can be an efficient way to improve beer quality. The objective of this study was to understand the effect of trxS gene on hydrolases activities in transgenic and non-transgenic barley seeds. The results showed that α-amylase, free β-amylase and limit dextrinase activity were increased in transgenic seeds in comparison with non-transgenic seeds. Sulfhydryl content of protein in transgenic seeds was also higher than that in non-transgenic seeds, suggesting that trxS gene could express in barley seeds, which opens a new way for breeding new barley varieties to improve beer quality.

关 键 词：TRXS基因 发芽 Α-淀粉酶 Β-淀粉酶 极限糊精酶 巯基 

分 类 号：Q943.2[生物学—植物学] S512.3[农业科学—作物学]