赭曲霉毒素A无毒体系胶体金试纸条的研制及与传统胶体金试纸条的比较  

Development of Colloidal Gold Strip with Mimotope Peptide for Rapid Detection of Ochratoxin A and Comparison between It and Traditional Strip

作　　者：赖卫华[1] 许杨[1] 熊勇华[1] 陈媛 刘文娟 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]江西中德生物工程有限公司,江西南昌330029

出　　处：《食品科学》2008年第9期465-468,共4页Food Science

基　　金：教育部“长江学者和创新团队发展计划”项目(IRT0540);国家科技型中小企业创新基金项目(06C26213601361);江西省科技厅社会发展攻关重点项目(2006)

摘　　要：本实验介绍了赭曲霉毒素A无毒体系胶体金试纸条的研制方法,其中包括胶体金的生产、金标抗体的制备、无毒体系试纸条的组装等步骤。所制备的赭曲霉毒素A无毒体系胶体金试纸条和传统的胶体金试纸条进行了比较,测试结果表明,赭曲霉毒素A无毒体系胶体金试纸条的检测限和检测时间与传统的胶体金试纸条相同,适合于赭曲霉毒素A现场筛查之用。A method for rapid detection of ochratoxin A by colloidal gold strip with mimotope peptide was developed. Three steps were involved in the process of the preparation of the strip, including production of colloidal gold, manufacture ofimmuno gold and installation of the strip. Compared with the traditional strip, the strip with mimotope peptide has the same limit and time of detection. The method using mimotope peptides instead of mycotoxin is suitable to screen ochratoxin A on site.

关 键 词：赭曲霉毒素A 胶体金:试纸条 模拟表位肽 

分 类 号：TS207.7[轻工技术与工程—食品科学]