杭白菊挥发油成分分析及β-榄香烯含量的测定  

Composition Analysis of Volatile Oil Extracted from Chrysanthemum moriflium Ramat and Determination of β-Elemene

作　　者：孙桂菊[1] 张菲菲[2] 马永建[3] 杨立刚[1] 康学军[1] 王少康[1] 

机构地区：[1]东南大学公共卫生学院营养与食品卫生系,江苏南京210009 [2]复旦大学医学院附属妇产科医院,上海200011 [3]江苏省疾病预防控制中心,江苏南京210009

出　　处：《食品科学》2008年第9期506-510,共5页Food Science

基　　金：江苏省科技攻关计划项目(BE2006318)

摘　　要：目的:分析杭白菊挥发油的化学成分并建立杭白菊中β-榄香烯的测定方法。方法:利用气相色谱-质谱联用仪分析杭白菊挥发油各组成成分,并使用气相色谱内标法测定杭白菊中β-榄香烯的含量。结果:杭白菊挥发油经气相色谱-质谱联用仪分析,共分离出125个峰,鉴定出59种化合物,占挥发油总量的61.74%;经分析,β-榄香烯线性范围为0.006～0.096mg/ml,RSD为0.85%,平均回收率为95.23%,在杭白菊中含量为12.98mg/kg。结论:利用气-质联用分析技术鉴定出菊花挥发油中的59种化合物,主要成分为单萜烯类、倍半萜烯类及其含氧衍生物等,杭白菊中具有抗肿瘤活性物质β-榄香烯的含量为12.98mg/kg。Objective： To analyze the ingredients in volatile oil extracted from Chrysanthemum moriflium Ramat and to determine the content of β-elemene in the volatile oil. Method： GC- MS analysis was used to analyze the composition of the volatile oil and the ninner standard method was used to determine the content of β-elemene. Results ： 59 compounds among 125 peaks separated out by GC-MS were indentified, accounting for 61.74% of total quantity of the volatile oil. The linear range of β- elemene is from 0.006 mg/ml to 0.096 mg/ml. The relative standard derivation（RSD） of repeated experiment is 0.85 %. The average recovery is 95.23 %. And the content of β-elemene in chrysanthemum moriflium Ramat is 12.98 mg/kg. Conclusions： The analysis of main composition of volatile oil from Chrysanthemum moriflium Ramat by GC- MS can enrich the study on constituents of this plant in the world and lay the foundation for further utilization. The internal standard method for determine the content of β-elemene in the volatile oil is simple, accurate, reproducible and practical. This study can promote development and utilization of traditional Chinese herbal medicines.

关 键 词：杭白菊 挥发油 分析 Β-榄香烯 

分 类 号：R151.3[医药卫生—营养与食品卫生学]