Simultaneous determination of four active compounds in Dangguilonghui tablet by high-performance liquid chromatography  

高效液相色谱法同时测定当归龙荟片中四种活性成分的含量(英文)

作　　者：胡春丽[1] 王冬梅[1] 张鹏[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《Journal of Chinese Pharmaceutical Sciences》2008年第3期249-253,共5页中国药学（英文版）

摘　　要：A high-performance liquid chromatography （HPLC） method has been developed for the first time to simultaneously quantify the four active ingredients, namely aloha, baicalein, aloe-emodin and wogonin, in Dangguilonghui tablet. The marker compotmds were separated on the Diamonsil C18 column at a flow rate of 1.0 mL/min with a mobile phase of methanol-acetonitrile-0.3% aqueous phosphoric acid （220： 4： 200, v/v/v） and while the detection wavelength was set at 225 nm. The assay was linear over the range of 1.450 - 29.00 ug/mL （r = 0.9992） for aloin, 0.4050 - 8.100ug/mL （r = 0.9994） for baicalein, 0.1100 - 2.200 ug/mL （r = 0.9997） for aloe-emodin, 0.2160 - 4.320 ug/mL （r = 0.9991） for wogonin, respectively. The average sample recoveries at three concentration levels were 100.7% （RSD = 0.88%）, 101.0% （RSD = 0.89%）, 100.0% （RSD = 1.3%） and 100.1% （RSD = 1.1%） for these constituents, respectively. This method is suitable for the quality control of Dangguilonghui tablet.建立同时测定当归龙荟片中芦荟苷,黄芩素,芦荟大黄素和汉黄芩素的含量的HPLC方法。采用Diamonsil C_(18)柱(200.0 mm×4.6 mm,5μm)分离,以甲醇-乙腈体积分数为0.3%磷酸溶液(220:4:200,v/v/v)为流动相进行沈脱,流速为1.0 mL/min,检测波长为225 nm。芦荟苷的线性范围为1.450～29.00μg/mL(r=0.9992),黄芩素的线性范围为0.4050～8.100μg/mL (r=0 9994),芦荟大黄素的线性范围为0.1100～2.200μg/mL(r=0.9997),汉黄芩素的线性范围为0.2160～4.320μg/mL (r=0.9991)。方法的平均回收率分别为100.7%(RSD%=0.88%),101.0%(RSD%=0.89%),100.0%(RSD=1.3%)和100.1% (RSD=1.1%)。为当归龙荟片的质量控制提供了科学的方法。

关 键 词：Dangguilonghui tablet ALOIN BAICALEIN ALOE-EMODIN WOGONIN HPLC 

分 类 号：R286.0[医药卫生—中药学]