无溶剂体系中脂肪酶催化大豆油水解反应的研究  

Lipase catalyzed hydrolysis of soybean oil in solvent-free system

作　　者：刘亚轩[1] 金青哲[1] 刘元法[1] 王兴国[1] 

机构地区：[1]江南大学食品学院食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《中国油脂》2008年第10期32-36,共5页China Oils and Fats

基　　金：国家“十一五”支撑计划项目(批准号:20060BAD27B03);教育部博士点基金项目(批准号:20070295004)资助

摘　　要：对无溶剂体系中C.lipolytica脂肪酶催化大豆油水解的反应条件进行了研究。在单因素实验的基础上,应用中心组合旋转设计原理对反应温度、pH、加酶量、水油质量比4个因素进行了5水平的实验设计,对实验结果进行响应面分析,得到回归方程。对回归结果进行了方差分析和典型分析,确定了响应面的驻点值,并对模型预测结果用实验方法进行了验证。Reaction conditions for lipase catalyzed hydrolysis of soybean oil in solvent - free system were investigated. Based on the single factor experimental results of temperature, pH, enzyme dosage and mass ratio of water to oil, a central composite rotatable design method was use to design experiments. The experimental results were analyzed by response surface method, and a regression equation was obtained. Variance and canonical analysis were used to explain the regression model. Stationary point of response surface was located. Additionally, the model was proved by experiments.

关 键 词：脂肪酶 大豆油 水解 响应面分析法 

分 类 号：TS225.13[轻工技术与工程—粮食、油脂及植物蛋白工程]