用蛹虫草固体发酵法高效生产虫草素的研究  

Investigations on cordycepin production by solid culture of Cordyceps militaris

作　　者：韦会平[1,2] 叶小莉[3] 张华英[2] 李学刚[1] 钟运俊[3] 

机构地区：[1]西南大学药学院,重庆400716 [2]攀枝花学院医学院,四川攀枝花617000 [3]西南大学生命科学学院,重庆400716

出　　处：《中国中药杂志》2008年第19期2159-2162,共4页China Journal of Chinese Materia Medica

基　　金：重庆市卫生局医学科技项目(渝中医[2001]25号)

摘　　要：目的：研究并建立用蛹虫草固体发酵方式高效生产虫草素的最佳方法。方法：通过对不同菌株在固体发酵过程中各生长期的虫草素含量变化进行跟踪测定以及正交旋转组合设计、正交试验设计等方法，影响虫草素产量和含量的各影响因子（包括菌种、生长期、培养基配方和环境条件等）进行系统优化。结果：JF-1是虫草素生产的最佳菌株，现蕾期是收获提取虫草素的最佳时期；最佳的培养基配方为水料比1．1：1（mL·g^-1营养水中酵母膏、蛋白胨、葡萄糖的用量分别为22．6，6．0，25．4g·L^-1水pH6．6；最佳的环境条件为：光照强度4400lx，每13光照时间18h，温度18—22℃。结论：用以上方法固体发酵生产虫草素，经过约13d的培养，培养基中虫草素达到0．60％，比传统液体发酵方法的最高产量高近2倍，并且培养时间缩短2d。An efficient method to produce cordycepin by solid culture using Cordyceps militaris was investigated in this study. Firstly, the changes of cordycepin during various growing periods of solid culture using 5 strains of C. militaris were detected, the best strain and optimal growing period for cordycepin production were determined. Then, by experiments of quadratic rotation-orthogonal combination design and orthogonal design,the medium composition and growth conditions for high yield of cordycepin were optimized. With the optimized method to produce cordycepin,the content of cordycepin in the medium was increased to 0.60%, which was nearly 2 times higher than the highest yield reported.

关 键 词：虫草素 蛹虫草 固体发酵 生物制药 

分 类 号：S567.35[农业科学—中草药栽培]