高效液相色谱法检测大豆酸乳中微量异黄酮的研究  

Determination of trace isoflavones in soybean yoghurt by high performance liquid chromatography

作　　者：李晓磊[1] 李丹[1] 高长城[1] 陈星[1] 

机构地区：[1]长春大学吉林省普通高等学校农产品加工重点实验室,吉林长春130022

出　　处：《长春大学学报》2008年第10期61-64,共4页Journal of Changchun University

基　　金：国家科技成果重点推广计划项目(2003EC000127);吉林省科技发展计划项目(20050125)

摘　　要：大豆酸乳不仅水分含量高，而且存在蛋白质、糖分和增稠剂等干扰物质，采用常规方法很难准确定量异黄酮。本研究以喷雾干燥的方法对大豆酸乳进行前处理，再以甲醇超声波提取乳粉中的异黄酮，然后采用高效液相色谱仪，以C18色谱柱为分离柱和甲醇／杉甲酸为流动相，在30min内，以0．8mL/min的流速梯度洗脱（从20／80／0．1到80／20／0．1）大豆酸乳粉的甲醇提取物，紫外检测器的波长设为254nm。结果表明：大豆酸乳粉中主要的异黄酮为染料木苷和大豆苷，总异黄酮含量为0．000544％～0．000937％。结论：利用这种方法可以准确的检测大豆酸乳中微量异黄酮含量．检出限大于百万分之一。It is difficult to determine the isoflavones in soybean yoghurt accurately by conventional methods, not only because of the high moisture, but also the interference substances such as protein, sugar and thickener. Spray drying was adopted to pretreat soybean yoghurt. Methanol and ultrasonicwave were used to extract the isoflavones from the flour. The sample was analyzed by a high performance liquid chromatography （HPLC） system equipped with a UV detector at 254 nm. Mobile phase from 100% methanol/water/formic acid （20/80/0. 1 ）to 100% methanoL/water/formic acid（80/20/0. 1 ）in 30 rain was used to elute isoflavones at 0. 8mL/min in a C18 column. The result show that daidzin and genistin were identified as the main isoflavones and occupied 0. 000544% - 0. 000937% in the soybean yoghurt flour. The conclusion is using this method can determine the isoflavones in soybean yoghurt accurately and the detection limit is more than 1/10^6.

关 键 词：大豆酸乳 异黄酮 高效液相色谱 喷雾干燥 

分 类 号：TS201[轻工技术与工程—食品科学]