超高压物理能对胡萝卜汁中类胡萝卜素异构体的影响  

Effect of Ultra-high Pressure Physical Energy on Carotenoid Isomers in Carrot Juice

作　　者：马永昆[1] 徐丽娜[1] 张佳[1] 上官丽娟[1] 李祥波[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品科学》2008年第10期105-108,共4页Food Science

基　　金：国家"863"计划项目(2007AA100405)

摘　　要：本实验研究了超高压处理对胡萝卜汁中类胡萝卜素异构体的作用,为利用超高压技术调控利于人体吸收的类胡萝卜素几何异构体奠定基础。胡萝卜汁分别进行100、300、500、700MPa处理5min,然后用C30柱加之二极管阵列检测器的HPLC分离检测样品中类胡萝卜素几何异构体。根据类胡萝卜素组分的色谱行为、光谱特征及Q值对其异构体进行定性,确定主要的类胡萝卜素有全反式β-胡萝卜素、15-顺-、13-顺-、9,15-顺-、9-顺-β-胡萝卜素和全反式α-胡萝卜素等。结果表明,超高压处理可使能垒较低的9、13、15位等全反式类胡萝卜素发生部分顺式异构化或降解生成小分子片段。Carrot juice was pressurized under different ultra-high pressure processing conditions and the aim was to study the effects of ultra-high pressure physical energy on the carotenoid geometric isomers in the carrot juice. The carotenoid geometric isomers in the juices treated by 100, 300, 500 and 700 MPa for 5min were separated respectively by HPLC equipped with a photodiode array detector and a C30 column. According to the chromatographic behavior, spectral characteristics and geometrical transition of the carotenoid geometric isomers catalyzed by iodine, the isomers of all -trans- β -carotene ,15-cis-,13-cis-, 9-15- di-cis-,9-cis- β-carotene, and all -trans- α -carotene were identified respectively. The results showed that to some extents, the ultra high pressure physical energy can lead to the isomerization of all-trans-carotenoids such as 9-, 13-, 15- carotenoids with low bond energies or the degradation to some small molecular fragments.

关 键 词：超高压物理能 HPLC C30柱 胡萝卜素异构体 

分 类 号：TS201[轻工技术与工程—食品科学]