非水相中脂肪酶催化合成乙酸薄荷酯  

Enzymatic synthesis of menthyl acetate in nonaqueous medium

作　　者：曾家豫[1,2] 唐功[1] 周兴辉[1] 高亚娟[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]甘肃省高分子材料重点实验室,甘肃兰州730070

出　　处：《食品工业科技》2008年第11期232-234,247,共4页Science and Technology of Food Industry

基　　金：甘肃省教育厅科研项目(0601-28;0501B-16)

摘　　要：研究了脂肪酶(candida rugosa lipase,CRL)在有机溶剂中催化薄荷醇与乙酸进行酯化反应的条件。探讨了脂肪酶加入量、溶剂、薄荷醇与乙酸摩尔比、底物浓度、温度等因素对脂肪酶催化合成乙酸薄荷酯的影响,并利用红外光谱、GC-MS、气相色谱等方法对合成的产物进行了表征。结果显示,合成的产物为乙酸薄荷酯。当溶剂为正己烷、脂肪酶量为1.2%(脂肪酶在薄荷醇和乙酸体系中的质量分数)、薄荷醇与乙酸的摩尔比为1∶1、薄荷醇与乙酸的底物浓度均为0.1mol/L、温度为40℃、反应48h时,乙酸薄荷酯的酯合成转化率达92.4%。产物乙酸薄荷酯的色泽好、气味纯正。The esterification conditions between acetic acid and menthyl alcohol, which was catalyzed by lipase （candida rugosa lipase, CRL） in organic solvents, was investigated. The effects of the lipase amount, solvent, molar ratio of menthyl alcohol to acetic acid, substrate concentration and the temperature on the esterification were discussed. The results indicated that the esterification conversion reaches to 92.4% ,when the reaction was carried out 48h in n-hexane, lipase amount was 1.2% （lipase mass fraction in the menthyl alcohol and acetic acid system） ,the molar ratio of menthyl alcohol to acetic acid was 1：1,the concentration both of the substrate was 0.1mol/L,and the temperature was 40℃. The product of menthyl acetate＇ s color was good and the odor was pure.

关 键 词：酶催化酯化反应 非水相介质 乙酸薄荷酯 

分 类 号：TS202.3[轻工技术与工程—食品科学]