高郊液相色谱对保健食品中五种违禁的性功能药物成分的同时检测  

Simultaneous determination of five species of contraband medicine component of enhance sexual function in health food by HPLC

作　　者：魏强[1] 万春花[2] 龙洲雄[2] 胡海山[2] 范青生[1] 

机构地区：[1]南昌大学食品科学和生物技术国家重点实验室,中德联合研究院,江西南昌330047 [2]江西省分析测试研究所,江西南昌330029

出　　处：《食品工业科技》2008年第11期260-264,共5页Science and Technology of Food Industry

摘　　要：目的:建立一种同时测定保健食品中5种常见违禁添加的增强性功能药物成分的反相液相色谱方法。用二极管阵列检测器检测,利用色谱峰保留时间和物质的紫外吸收光谱综合定性,外标法定量。方法:采用反相高效液相色谱法实现5种成分完全分离。Agilent C184.6×250mm色谱柱,柱温30℃,流动相为乙腈/0.02%磷酸,梯度洗脱,流速1.0mL/min,检测波长为225.0nm。结果:5种违禁性功能药物成分在0.4～20.0μg/mL浓度范围内工作曲线线性关系良好,相关系数r2≥0.9989,相对标准偏差≤0.47%(n=6),平均回收率>91.0%,最低检出限≤56ng/mL(信噪比≥3)。结论:该方法简便快速,结果准确可靠,可用于保健食品中常见添加的性功能药物成分的测定。Objective：to establish a practical method of RP-HPLC for the simultaneous determination of five kinds of contraband medicine component to enhance sexual function in health food. Photodiode array detector was used, basing on chromatogram retention time and photodiode array spectrum for qualitative analysis and external standard method with peak area for quantitative analysis. Methods： the five components could be separated completely on a Agilent C18 column （4.6 × 250mm）and then detected at 225.0nm, using acetonitrile and 0.02% phosphoric acid with gradient elution as the mobile phase at flow rate 1.0mL/min, and the column temperature was 30℃. Results：the linear range of the calibration curve of five components were 0.4～20.0μg/mL （ r^2≥0.9989）, RSD no higher than 0.47% （n = 6）, the rates of average recovery was higher than 91.0%, the detection limits of five components were no higher than 56ng/mL （ S/N ≥3 ）. Conclusion： the method was simple, rapid, accurate and reliable that could be used to determine frequent contraband ingredient of enhance sexual function in health food.

关 键 词：高效液相色谱 保健食品 违禁药物成分 测定 

分 类 号：TS207.3[轻工技术与工程—食品科学]