海洋细菌Pseudoalteromonas sp．G23产低温淀粉酶发酵条件的研究  

Production conditions of cold-active α-amylase from marine bacteriun Pseudoalteromonas sp. G23

作　　者：王淑军[1] 刘红飞[1,2] 李华钟[2] 房耀维[1] 吕明生[1] 刘姝[1] 

机构地区：[1]淮海工学院食品工程学院,江苏连云港222005 [2]江南大学教育部工业生物技术重点实验室,江苏无锡214122

出　　处：《中国酿造》2008年第12期9-12,共4页China Brewing

基　　金：国家自然科学基金项目(40746030)

摘　　要：从江苏连云港海域筛选和分离到1株产低温淀粉酶的海洋细菌Pseudoalteromonas sp.G23,对该菌株进行了产α-淀粉酶发酵条件研究,结果表明,该菌株发酵16h后到达产酶高峰,最适产酶温度为15℃,最适产酶pH值为8.5,装液量为20%。可溶性淀粉、酵母膏和蛋白胨促进产酶。利用响应面方法对菌株Pseudoalteromonas sp.G23产α-淀粉酶的发酵条件进行了优化,结果表明,可溶性淀粉浓度为1.16%、蛋白胨浓度为2.11%,发酵温度12.38℃,酶活为41.4U/mL,较优化前提高了7倍。A cold-active amylase producing marine bacterium Pseudoalteromonas sp. G23 was screened and isolated from the sea area of Lianyungang, Jiangsu. The production of α-amylase from this strain has been studied. Maximum enzyme production was achieved after 16h cultivation. The optimum temperature, pH and medium volumn were 15℃, 8.5 and 20%, respectively. The addition of soluble starch, yeast extract and peptone enhanced enzyme production. The response surface design was used to optimize the fermentation conditions for α-amylase production by Pseudoa teromonas sp. G23. The optimum conditions were soluble starch 1.16%, peptone 2.11%, and fermentation temperature 12.38℃. After optimizatiol the amylase activity was 41.4 U/ml, which was increased by 7 times compared with that before optimization.

关 键 词：海洋细菌 PSEUDOALTEROMONAS sp. 低温淀粉酶 发酵优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]