Identification of Tumor Progression-Related Genes in Astrocytoma Cell Lines  

作　　者：Yi Zeng Zhong Yang Chao You Yangyun Han 

机构地区：[1]Department of Neurosurgery, People＇s Hospital of Deyang City, Deyang 618000, Sichuan Province, China [2]Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China [3]Department of Neurobiology, Third Military Medical University, Chongqing 400038, China

出　　处：《Chinese Journal of Clinical Oncology》2008年第6期391-399,共9页中国肿瘤临床（英文版）

基　　金：This work was supported partly by a grant from the Sichuan Provincial Bureau of Health,China(No.050209)

摘　　要：OBJECTIVE To identify progression-related genes that lead toastrocytoma progression from a low to a high grade by analyzingthe gene expression profiles from different tumor grades,and all-trans retinoic acid-treated astrocytoma cell lines.METHODS In this study,all-trans retinoic acid (ATRA) wasused to induce differentiation of SHG-44 cells.Then,by usinga cDNA microarray,we compared gene expression profilingin different grades of astrocytoma cell lines (CHG-5,WHOGrade Ⅱ and SHG-44,WHO Grade Ⅳ) and in ATRA-induceddifferentiation in SHG-44 cells.A panel of overlapped genes thatmight be related to tumor progression was identified,and the cellline individual variation was avoided as well.RESULTS In the 31 overlapped genes,the stable over-expressionof MDM2 and UGB as well as the repression of SOD2,G3BP,andCSTB in parental SHG-44 cells was observed and their possibleroles in tumor progression were discussed.Moreover,validationof some differentially expressed genes was confirmed by Northernblots.CONCLUSION The overlapped genes reported in this studymight relate to progression of astrocytoma.Further study ofprogression-related genes may be helpful to explore the geneticpathways that are involved in astrocytoma progress from a low toa high grade.OBJECTIVE To identify progression-related genes that lead to astrocytoma progression from a low to a high grade by analyzing the gene expression profiles from different tumor grades, and all- trans retinoic acid-treated astrocytoma cell lines. METHODS In this study, all-trans retinoic acid （ATRA） was used to induce differentiation of SHG-44 cells. Then, by using a cDNA microarray, we compared gene expression profiling in different grades of astrocytoma cell lines （CHG-5, WHO Grade Ⅱ and SHG-44, WHO Grade Ⅳ） and in ATRA- induced differentiation in SHG-44 cells. A panel of overlapped genes that might be related to tumor progression was identified, and the cell line individual variation was avoided as well. RESULTS In the 31 overlapped genes, the stable over-expression of MDM2 and UGB as well as the repression of SOD2, G3BP, and CSTB in parental SHG-44 cells was observed and their possible roles in tumor progression were discussed. Moreover, validation of some differentially expressed genes was confirmed by Northern blots. CONCLUSION The overlapped genes reported in this study might relate to progression of astrocytoma. Further study of progression-related genes may be helpful to explore the genetic pathways that are involved in astrocytoma progress from a low to a high grade.

关 键 词：PROGRESSION CHG-5 SHG-44 ASTROCYTOMA cDNA microarray. 

分 类 号：R730.21[医药卫生—肿瘤]