气相色谱-质谱法测定苦瓜超临界CO_2流体萃取产物的挥发性成分  

Analysis of Volatile Components from Momordica Charantia L. by Gas Chromatography/Mass Spectrometry with Supercritical CO_2 Fluid Extraction

作　　者：贾素花[1] 胡志国[1] 李昌[1] 张中伟[1] 陈振桂[1] 王远兴[1] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330031

出　　处：《南昌大学学报（理科版）》2008年第5期479-482,共4页Journal of Nanchang University(Natural Science)

基　　金：教育部长江学者和创新团队发展计划资助项目[IRT0540]

摘　　要：采用超临界CO2萃取法(SFE)从苦瓜中提取有效成分,萃取条件为:萃取釜压力25 MPa,温度42℃,萃取时间3 h,夹带剂无水乙醇用量20 mL。采用气相色谱-质谱法对其挥发性成分进行鉴定,用归一化法测定各组分的含量。色谱条件:HP-5MS石英毛细管柱(0.25 mm*30 m*0.25μm),柱温60℃,恒温1 min,以10℃/min升至280℃,保持10 min,分流进样,分流比20:1。进样口温度250℃。在采用超临界CO2萃取法提取的苦瓜提取物中共检出20种成分。Volatile components were extracted from Momordica charantia L. by Supercritical CO2 fluid extraction . Extraction condition：extracting 3 h at 42 ℃ ,25 MPa （pressure） and using 20 mL alcohol as modifier. The volatile components extracted were determined by gas chromatography with area normalization method and identified by gas chromatography mass spectrometry （GC - MS）. The optimal chromatographic conditions were：HP -5 MSsilica capillary column （0.25 mm ＊ 30 m ＊ 0.25 μm） ;column temperature：60 μ （ hold for 1 min） to 280 ℃ at a rate of 10 ℃/min, and hold for 5 min. split injection,20 ： 1 ; injector temperature,250 ℃. Twenty component of the extracted from Momordica charantia L. were identified by GC - MS.

关 键 词：超临界CO2 萃取 气相色谱-质谱 苦瓜 

分 类 号：O658[理学—分析化学]