基质固相分散-液相色谱法测定火腿肠中酚类环境雌激素  

Determination of Phenolic Environmental Estrogens in Ham Sausage with Matrix Solid Phase Dispersion-Liquid Chromatography

作　　者：周建科[1] 赵瑞峰[1] 王立双[1] 

机构地区：[1]河北大学理化分析中心,河北省分析科学技术重点实验室,河北保定071002

出　　处：《食品科学》2008年第12期528-530,共3页Food Science

摘　　要：硅酸镁(florisil)作固相分散剂与样品混合,正己烷脱油脂,二氯甲烷洗提三种酚类环境雌激素,高效液相色谱法测定。色谱条件为:Diamonsil C18色谱柱(5μm,250mm×4.6mm),乙腈-甲醇-水(20:43:37,V/V)为流动相,甲酸调节pH=5。双酚A、己烯雌酚、己二烯雌酚在0.0005～0.1mg/ml之间均呈良好线性关系,样品检出限分别为0.05、0.1、0.05μg/g(S/N=3),相对标准偏差(RSD)低于4.5%,回收率80%以上。Matrix solid phase dispersion was used to treat three phenolic environmental estrogens in sample （Florisil used as dispeisant, hexane used as degreaser, and dichloromethane used as eluent for the target compounds）. These phenolic compounds were determined with high performance liquid chromatography. A Diamonsil C18 （5 μ m, 250 mm × 4.6 mm） was used for separation. Mobile phase were composed of acetonitrile, methanol and water （the ratio of volume was 20：43：37 and pH was regulated to 5.0 with formic acid）. Bisphenol A, stilbestrol and dienestrol show good linearity in the concentration range of 0.0005 to 0.1 mg/ml, and the detection limits are 0.05, 0.1, 0.05 μg/g respectively （S/N=3）. This method has high precision and accuracy with recovery over 80% and relative standard deviations lower than 4.5%.

关 键 词：基质固相分散 液相色谱 火腿肠 酚类环境雌激素 

分 类 号：TS207.5[轻工技术与工程—食品科学]