拟康氏木霉固态发酵产纤维素酶系的研究  

Study of cellulose-production by Trichoderma pseudokoningii in solid fermentation

作　　者：王实玉[1] 陈雄[1] 王永泽[1] 王志[1] 李冬生[1] 

机构地区：[1]湖北工业大学生物工程学院,武汉市430068

出　　处：《中国饲料》2008年第23期23-26,共4页China Feed

基　　金：湖北省教育厅重点项目(D20081401);武汉市科技局重大项目(200820212029)

摘　　要：以稻草秸秆为主要原料,利用拟康氏木霉3.3002(Trichoderma pseudokoningii 3.3002)固态发酵生产纤维素酶,对培养条件进行了优化,并系统地测定了各种纤维素酶的酶活。结果表明,最优产酶培养基组成为:稻草秸秆和麸皮的混合比例为4∶1,最佳氮源为2.5%(NH4)2SO4,最佳发酵时间120h,培养温度35℃,接种量15%,pH5.0,培养基含水率50%。在此条件下,该菌株产纤维素酶系中羧甲基纤维素酶(Cx)酶活力达4700U/mL,葡聚糖外切酶(Cb)酶活力达3440U/mL,葡萄糖内切酶(C1)酶活力达到1620U/mL,滤纸酶(FPA)酶活力达到1935U/mL。Using the straw as the main substrate ,the optimal production conditions of cellulase by Trichoderma pseudokoningii 3.3002 in solid fermentation were investigated.The cellulase activity was also systematically measured.The optimal fermentation conditions showed as follows ：straw and bran mixture was used as fermentation medium （with ratio of 4： 1 ）, addition of 2.5 % （NH4）2SO4 with initializing medium adjusted to pH 5.0,the cultivate time of 120 h at 35 ℃, inoculating volume of 15 %.The water content of medium was 50 %.Under stated conditions,cellulase activity of Cx,Cb,C1 and FPA could reach 4700,3440,1620 U/mL and 1935 U/mL,respectively.

关 键 词：固态发酵 纤维素酶 最优产酶培养基 酶活力 

分 类 号：S816.3[农业科学—饲料科学]