醋酸铅试纸法在低产H_2S酿酒酵母筛选中的应用  

Application of lead acetate test paper in the screen of low-H_2S-producing yeast strains

作　　者：韩娜[1] 刘延琳[1] 刘爱国[1] 王泽举[1] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2009年第1期225-228,234,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"计划项目(2007AA10Z314);西北农林科技大学人才支持计划项目(01140301)

摘　　要：【目的】探讨醋酸铅试纸法筛选低产硫化氢(H2S)酿酒酵母的可行性。【方法】在模拟汁培养基发酵条件下,以采自新疆鄯善地区酿酒葡萄自然发酵液中的10株野生型酿酒酵母(M1,M2,…,M10)为对象,采用醋酸铅试纸法对其中的低产H2S酵母进行了初筛和复筛。【结果】经醋酸铅试纸初筛后,菌株M2、M3、M6属于低产H2S的菌株,而M1、M8、M10属于高产H2S的菌株。复筛结果显示,菌株M2、M3、M6的硫化氢生成量较低,均为12μg/L;菌株M10的H2S生成量最高,为1 920μg/L。模拟发酵试验表明,有8株野生型酿酒酵母(M7和M9除外)发酵正常,具备良好的发酵能力,酒精度都在10%(体积分数)以上。【结论】醋酸铅试纸法可用于低产H2S酿酒酵母的筛选,该法具有结果稳定,方法简便,成本低廉,操作简单等优点,有一定的推广价值。菌株M2、M3和M6皆为低产H2S酿酒酵母,且其发酵能力适合工业发酵要求。[Objective] The feasibility of the lead acetate test paper in screening of low-yield H2S yeast strains was studied. [Method] With 10 wild Saccharomyees cerevisiae yeast strains,separated from Shansban, Xinjiang province, as subject, low-yield H2S yeast strains were screened twice in the fermented simulated juice with the method of lead acetate test paper. [Result] The results showed that M2, M3, M6 were low-yield H2 S strains, M1, MS, M10 high-yield H2 S strains. Among them, M2, M3, M6 produced H2S 12 μg/L;M10 could produce the highest content of H2S by 1920μg/L. With higher ethanol-production ability （all above 10%） 8 wild yeast strains （except M7 and M9） showed superior characters in the fermentation experiment. [Conclusion] The method of lead acetate test paper is convenient, low-cost, stable, and has high value for expansion and application in the screen of low-yield H2S yeast strains. With the method, the three yeast strains suitable for industrial fermentation of M2, M3, M6 were screened as low-yield H2S strains.

关 键 词：醋酸铅试纸法 酿酒酵母 低产硫化氢 

分 类 号：TS261.1[轻工技术与工程—发酵工程]