caspase-3在慢性粒细胞白血病信号转导中的作用  

Role of caspase-3 in the signal transduction of chronic myelogenous leukemia

作　　者：尚振川[1] 宋霆婷[2] 孙秉中[3] 侯严堂[1] 冯琦[3] 张涛[3] 邓涛[1] 

机构地区：[1]成都军区总医院血液科,成都市610083 [2]青岛市肿瘤医院 [3]第四军医大学西京医院血液科

出　　处：《中华实用诊断与治疗杂志》2009年第1期44-46,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：两项国家自然科学基金课题资助(No.39670330和No.39770336)

摘　　要：目的:探讨caspase-3在慢性粒细胞白血病信号转导中的作用。方法:用脂质体转染法将bcr3/abl2ASO导入K562细胞中,通过检测细胞凋亡、caspase-3表达和caspase-3活性的变化,观察caspase-3在慢性粒细胞白血病信号转导中的作用。结果:bcr3/abl2ASO可诱导K562细胞凋亡;正常情况下caspase-3在K562细胞中有一定量的基础表达和活性,分别为(1.13±0.1)和(0.025±0.001);而K562发生凋亡时,caspase-3水平明显升高,其表达和活性分别为(2.93±0.6)和(0.903±0.003)。结论:caspase-3通过介导凋亡信号而在慢性粒细胞白血病信号转导中起重要作用。Objective To study the rote of caspase-3 in the signal transduction of chronic myelogenous leukemia. Methods After bcr3/abl2 antisense oligodeoxynucleotide was introduced into K562 cell line by liposomal transfection, the effect was evaluated by apoptosis, caspase-3 expression and caspase-3 activity. Results bcr3/abl2 antisense oligodeoxynucleotide can introduce the apoptosis of K562 cell line. The expression and activity of caspase-3 were （1. 13±0. 1） and （0.025±0.001） in normal K562 cell while those were （2.93±0.6） and （0. 903±0. 003） when K562 cell in apoptosis. Conclusion caspase 3 plays an important role in the signal transduction of chronic myelogenous leukemia by directing apoptotic signal transduction.

关 键 词：慢性粒细胞白血病 反义寡核苷酸 CASPASE -3 信号转导 

分 类 号：R733.72[医药卫生—肿瘤]