CD/5-FC自杀基因体系对肝、胆、胰肿瘤细胞的杀伤作用及机制  

Anticancer effect of cation lipofectin-mediated CD/5-FC suicide gene system on liver, biliary duct and pancreas carcinoma cells in vitro

作　　者：刘国彦[1] 罗琪[1] 庄维纯[1] 杨素梅[1] 魏黎煜[1] 

机构地区：[1]厦门大学附属中山医院普外科厦门大学消化疾病研究所,福建省厦门市361004

出　　处：《世界华人消化杂志》2008年第35期3946-3952,共7页World Chinese Journal of Digestology

基　　金：福建厦门市卫生局基金资助项目;No.wsk0509~~

摘　　要：目的:通过CD/5-FC自杀基因体系对肝BEL-7402、胆QBC、胰BXPC-3三系肿瘤细胞杀伤效率的比较,探讨影响这种杀伤差异的可能机制.方法:测定BEL-7402、QBC、BXPC-3三系肿瘤细胞的生长周期及倍增时间.FACS测定阳离子脂质体介导的三系肿瘤细胞瞬时转染效率.MTT法测定阳离子脂质体介导CD/5-FC自杀基因体系对瞬时转染的肝胆胰三系肿瘤细胞体外杀伤效率.观察细胞倍增时间与转染效率与杀伤效率之间的关系.FACS对三系肿瘤细胞瞬时转染自杀基因后凋亡进行比较,并用Hochest33342染色后进行观察.结果:BEL-7402、QBC、BXPC-3的倍增时间分别是34.48、64.94、26.29h.QBC细胞同BEL-7402及BXPC-3相比差异有显著性(P<0.05);BEL-7402、QBC、BXPC3三系细胞阳离子脂质体介导的转染率分别为26.99%、2.25%、30.36%.BEL-7402和BXPC-3同QBC细胞有显著差异(P<0.05);瞬时转染自杀基因后抑制效率分别为83.24%,16.97%,92.32%;在CD/5-FC杀伤三系细胞可能的机制-凋亡的研究中,三者凋亡率分别为27.8%,5.49%,36.5%.结论:生长周期短,倍增时间快的细胞系瞬时转染率高,对CD/5-FC自杀基因疗法比较敏感,该自杀基因疗法可能对临床晚期消化系恶性肿瘤是一种很有前景的治疗方法.AIM： To investigate the anticancer effect of the CD/5-FC suicide gene system on carcinoma cells of liver （Be17402）, bililary duct （QBC） and pancreas （Bxpc3）, and to explore the possible anticancer mechanism. METHODS： The growth curve and multiplication time of Bel-7402 QBC and Bxpc-3 were monitored by MTT; the transfection efficiency of the three kinds of carcinoma cells were detected by FACS after instant transfection. The anticancer efficiency of cation lipofectin-mediated CD/5- FC suicide gene system were monitored by MTT in vitro. Then, we analyzed the relation among multiplication time, transfection efficiency and anticancer effect; the apoptosis of CD/5-FC on the three cell lines were analyzed by FAM, and the apoptosis cells were stained by Hochest33342. RESULTS： The multiplication time of Bel-7402, QBC and Bxpc-3 was 34.48, 64.94 and 26.29 h respectively, and difference between QBC and BEL-7402, BXPC-3 was significant （P 〈 0.05）; the transfection efficiency of the three cell line were 26.99%, 2.25% and 30.36%, and the difference between QBC, and BEL-7402 and BXPC-3 was significant （P 〈 0.05）; the anticancer efficiency was 83.24%, 16.97% and 92.32%, respectively. The apoptosis rates of the three kinds of carcinoma cells induced by CD/5-FC suicide gene system were 27.8%, 5.49% and 36.5%, respectively. CONCLUSION： Carcinoma cells with shorter growth cycle and less multiplication time have higher instant transfection efficiency, and are more responsive to CD/5-FC suicide gene therapy. This suicide gene therapy may be a promising method for later malignant tumor in clinic.

关 键 词：自杀基因 五氟胞嘧啶 胞嘧啶脱氨酶 瞬时转染 肝 胆管 胰腺 肿瘤 

分 类 号：R735[医药卫生—肿瘤]