枳实提取物中类黄酮的高效液相色谱法测定及其抗氧化作用研究  

Determination of Flavoniods in Citrus aurantium L.Extract by HPLC and its Antioxidant Activity

作　　者：焦士蓉[1,2] 黄承钰[2] 

机构地区：[1]西华大学生物工程学院,四川成都610039 [2]四川大学华西公共卫生学院,四川成都610041

出　　处：《时珍国医国药》2009年第1期207-209,共3页Lishizhen Medicine and Materia Medica Research

基　　金：四川省教育厅自然重点项目资助(No.07ZA117)

摘　　要：目的建立了枳实中柚皮苷、橙皮苷、新橙皮苷、芦丁、槲皮素含量的测定方法，同时测定了枳实提取物的抗氧化作用。方法采用HPLC法，色谱柱：Phenomenex GeminiC18柱（150mm×4．6mm，5μm），柱温37℃，流动相为乙腈-磷酸溶液，检测波长280，365nm，流速1．0m1／min。采用DPPH·测定枳实提取物的自由基清除能力。结果进样量范围：柚皮苷为1．143～5．717μg，橙皮苷为0．092～0．459μg，新橙皮苷为1．179～5．895μg，芦丁为0．024～0．118μg，槲皮素为0．079～0．395μg。柚皮苷、橙皮苷、新橙皮苷的平均回收率分别为99．604％，100．150％，100．249％。RSD分别为0．515％，4．390％，0．774％。枳实提取物对DPPH·清除作用是柚皮苷的17．6倍。结论HPLC测定法简便、准确，可用于测定枳实类黄酮含量，同时枳实提取物具有较强的自由基清除作用。Objective To establish an HPLC method for determination of naringin, hesperidin, neohesperidin, rutin and quercetin in Citrus aurantium L. , and to study antioxidant activity. Methods The samples were extracted with ethanol. The chromatographic conditions were as follows： Phenomenex Gemini C lS column （150 mm × 4.6 mm, 5 μm） , column temperature at 37℃, mobile phase of acetonitrile - phosphate solution, detection wavelength at 280nm or 365nm, flow rate 1.0ml/min. In addition, DPPH radical - scavenging was employed to measure antioxidant activities. Results The calibration curves were linear in the range of 1. 143 -5. 717 μg for naringin, 0. 092 ～ 0. 459μg for hesperidin, 1. 179～ 5. 895 μg for neohesperidin, 0. 024 -0.118μg for rutin and 0. 079～ 0. 395μg for quercetin. The average recoveries of naringin, hesperidin, neohesperidin were 99. 604% , 100. 150% and i00.249% respectively. RSD were 0. 515% , 4. 390% and 0.774% respectively. The DPPH radical scavenging activity of C. aurantium L. flavoniods was 17.6 times of naringin. Conclusion The method is simple, accurate for detecting flavoniods in C. aurantium L. , and the C. aurantium L . extract has antioxidant activities.

关 键 词：枳实 类黄酮 高效液相色谱法 抗氧化作用 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]