γ-聚谷氨酸高产菌株Bacillus natto S003-D16的选育和优化  

Screening of high yield strain bacillus Natto S003-D_(16) producing γ-Polygluamic acidand its optimization of fermentation conditions

作　　者：张姝[1] 李楠[1] 黄登禹 王雷[1] 宋振玉[1] 徐振衣[1] 李树[1] 杨卿[1] 

机构地区：[1]天津市食品生物技术重点实验室天津商业大学生物技术与食品科学学院,天津300134 [2]天津百奥生物技术有限公司,天津300350

出　　处：《中国酿造》2009年第1期70-73,共4页China Brewing

摘　　要：以产γ-聚谷氨酸的纳豆芽孢杆菌(Bacillus natto S003)为出发菌,经紫外(UV)-硫酸二乙酯(DES)复合诱变,分离筛选得到1株高产稳定突变株Bacillus natto S003-D16,经摇瓶实验验证γ-聚谷氨酸的含量达到20.58g/L,较出发菌株提高40.38%。通过正交试验优化的培养基组成为:味精废液120mL/L、豆粕50g/L、葡萄糖30g/L、FeCl3 0.4g/L、MgSO4 0.6g/L、MnSO4 0.01g/L、K2HPO4 0.2g/L,pH7.0;利用优化的培养基在500mL三角烧瓶装液量100mL,37℃、230r/min条件下培养72h,发酵液中的γ-PGA产量可达到31g/L。纯化后产物经红外光谱鉴定其结果与标准品的图谱基本一致。A stable mutant Bacillus natto S003-D16 highly produced γ-polyglutamic acid was obtained after mutated by UV and DES compound mutation using Bacillus natto S003 as original strain. The γ-PGA yield was enhanced from 14.66 g/L to 20.58 g/L, which was increased by 40.38% compared to the original strain, The optimum compositions of fermentation medium determined by orthogonal experiments were as follows： Monosodium glutamate （MSG） waste liquid 120 ml/L, defatted soybean 50g/L, glucose 30g/L, FeCl3 0.4g/L, MgSO4 0.6g/L, MnSO4 0.01g/L, K2HPO4 0.2g/L and pH 7.0. The optimal environmental conditions were as follows： flask volume 100 ml of 500ml flask, temperature 37℃, pH 7.0, rotation speed 230 r/min and fermentation time 72h, Under these optimal conditions, the production of γ-PGA reached 31g/L. The purified product determined by Fourier transform infrared spectroscopy was consistent with the standard sample of γ-PGA.

关 键 词：Γ-聚谷氨酸 纳豆芽孢杆菌 复合诱变 优化 味精废液 

分 类 号：Q93-331[生物学—微生物学]