高产S-腺苷甲硫氨酸酵母菌株的筛选  

Isolation of Yeast Strains for S-adenosyl-L-methionine Production

作　　者：刘勇[1,2] 刘建玲[1] 王璋[2] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]中国食品发酵工业研究院,北京100027

出　　处：《食品与发酵工业》2008年第12期62-64,共3页Food and Fermentation Industries

摘　　要：从土壤和酒曲中对产S-腺苷甲硫氨酸的酵母菌株进行了筛选,通过HPLC外标法测定得到8株产量较高的酵母菌株。对高氯酸细胞破壁和酵母细胞水解作用条件进行优化,确定了高氯酸提取S-腺苷甲硫氨酸的理想条件为2.5mol/L高氯酸10mL,时间为5h,菌体量为0.6g。The yeast strains producing S-adenosyl-L-methionine （SAM） were isolated from the soil and solid koji samples. Eight yeast stains of higher SAM productivities were acquired with an external standard analytical method of HPLC. The best conditions of extracting SAM form the yeast ceils were 2.5mol/l perchloric acid 10ml, 5h hydrolytic time and 0.6g biomass by optimizing the condition between perchlorate and cells of yeast.

关 键 词：土壤 酒曲 S-腺苷甲硫氨酸 高氯酸 优化 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]