漆酶与水解酶协同改善OCC浆强度及纤维表面性能  

Synergistic Effect of Laccase and Hydrolase on OCC Pulp's Strength and Fiber's Surface Properties

作　　者：单旭[1] 裴继诚[1,2] 石淑兰[1] 孙兵[1] 王飞[1] 

机构地区：[1]天津科技大学天津市制浆造纸重点实验室,天津300222 [2]华南理工大学制浆造纸工程国家重点实验室,广州510641

出　　处：《纸和造纸》2009年第2期53-57,共5页Paper and Paper Making

基　　金：国家自然科学基金(30471359);制浆造纸工程国家重点实验室(华南理工大学)(200618);天津科技大学重点基金(20040108)资助课题

摘　　要：采用漆酶、纤维素酶和半纤维素酶,对旧瓦楞箱纸板(OCC)纸浆进行酶改性处理,研究了酶协同作用对OCC浆强度性能的影响。结果显示,当处理条件pH值7.3、温度45℃、浆浓3%、时间2h时,三种酶单独处理OCC浆料的最佳酶用量分别为:漆酶16U·g-1,通空气;纤维素酶2U·g-1;半纤维素酶5U·g-1。与空白样比较,采用纤维素酶、漆酶的两段处理,干环压指数提高27%,干、湿抗张指数分别提高18%和54%,湿环压指数提高14%。采用扫描电子显微镜(SEM)分析技术,对几种酶处理的OCC浆手抄片纤维表面形态变化进行了观察分析,结果表明,漆酶与纤维素酶或半纤维素酶协同作用处理样,纤维表面更加粗糙,纤维间黏合较漆酶单独处理增加。Old corrugated container （OCC） pulp was treated with laccase, cellulase and 1, 4-xylanase in our experiment. The results from a study of the effects on the strength characteristics of OCC caused by the three kinds of enzymes that were used in conjunction with each other show that：When handing the OCC under the conditions of pH 4.5, temperature at 45℃ and pulp concentration 3% for 2 hours, the optimum dosages of the three kinds of enzymes, used individually, were laccase 16U·g^-1 （with air injected）, cellulace 2U ·g^-1 and xylanase 5U ·g^-l. When treated under these conditions, wet tensile strength was increased effectively （by 87%）. The optimum sequence of enzymes was either xylanase at first, then laccase, or cellulase first and laccase last. Compared to the control, dry ring stiffness was increased by 27%, dry tensile strength by 18%, wet tensile strength by 54% and wet ring stiffness by 14%. According to fiber surface analysis by SEM, we have probed into the reason why the strength of the OCC obtained through cellulase and laccase treatment was higher compared to the bland, it is concluded that the fibers treated with cellulase and laccase or 1,4-xylanase and laccase in conjunction with each other had been modified to a greater extent than similar fibers that had been treated with laccase alone.

关 键 词：OCC浆 漆酶 纤维素酶 木聚糖酶 扫描电镜 

分 类 号：TS727.2[轻工技术与工程—制浆造纸工程]