绿色木霉ZY-1固态发酵产纤维素酶  

Cellulase production by Trichoderma viride ZY-1 under solid-state fermentation

作　　者：于莹[1] 姚日生[1,2] 齐本坤[1] 朱慧霞[1,2] 

机构地区：[1]合肥工业大学化学工程学院,合肥230009 [2]合肥工业大学农产品生物化工教育部工程研究中心,合肥230009

出　　处：《生物加工过程》2009年第1期54-58,共5页Chinese Journal of Bioprocess Engineering

基　　金：合肥工业大学科学研究发展基金资助项目(060604F)

摘　　要：利用筛选的绿色木霉ZY-1(Trichoderma virideZY-1)固态发酵产纤维素酶,采用稻草和麸皮为底物,考察稻草与麸皮比例随发酵时间对产酶的影响。结果表明:底物中,在m(稻草)∶m(麸皮)为0∶5和1∶4时,发酵48 h,pH保持4.5左右,还原糖量急剧上升,胞外蛋白产量最低;仅以稻草作底物时,整个发酵过程中pH约为7,还原糖量最低,胞外蛋白产量较高而滤纸酶活、羧甲基纤维素酶(CMCase)和β-葡萄糖苷酶(β-Gase)酶活均较低;在m(稻草)∶m(麸皮)为3∶2时,发酵96 h,滤纸酶活达最大值5.01 U/g干曲;m(稻草):m(麸皮)为1∶4时,发酵96 h,β-Gase酶活达最大值4.6 U/g干曲;m(稻草):m(麸皮)为4∶1时,发酵72 h,CMCase酶活达最大值6.01 U/g干曲。因此,底物中存在适量的稻草和麸皮有利于Trichoderma virideZY-1产纤维素酶。Cellulase was produced under solid-state fermentation by Trichoderma viride ZY-1 using wheat bran and straw as a substrate. The ratio effects of the rice straw to wheat bran on cellulase production was studied. The results showed that at higher concentration of wheat, bran, viz. the qualitative ratio of the rice straw to the wheat bran was 0：5 and 1 ： 4, after incubation for 48 h and pH remained at 4.5, the concentration of extracellular protein was the lowest, the concentration of reducing sugars was the highest, and activities of filter paper cellulase （ FPase）, carboxymethyl cellulase （ CMCase）, and β-glucosidase （β-Gase） were low. When rice straw was used as sole substrate and pH remained at 7, the concentration of reducing sugars was the lowest, but the concentrations of extracellular protein was high, the FPase, CMCase, and β-Gase activities were low. As the ratio of the rice straw to the wheat bran was 3： 2 and 1： 4, the activities of FPase and β-Gase reached the highest, i.e. 5.01 U/g dried substrate and 4.6 U/g dried substrate, respectively. As the ratio of the rice straw to the wheat bran was 4： 1, and fermentation time was 72 h, CMCase activity reached the highest at 6.01 U/g dried substrate.

关 键 词：纤维素酶 酶活 稻草 固态发酵 麸皮 

分 类 号：Q539.3[生物学—生物化学]