双波长法测定小麦及小麦芽中直链、支链淀粉含量  

Determination of Amylose and Amylopectin in Wheat and Wheat Malt by Dual-Wavelength Spectrophotometry

作　　者：金玉红[1] 张开利[2] 张兴春[2] 杜金华[1] 

机构地区：[1]山东农业大学食品科学与工程学院,泰安271018 [2]泰安泰山啤酒有限公司,泰安271000

出　　处：《中国粮油学报》2009年第1期137-140,共4页Journal of the Chinese Cereals and Oils Association

基　　金：山东省优秀中青年科学家科研奖励基金(编号20031B137)

摘　　要：采用双波长法对15种小麦品种的直、支链淀粉的含量进行了测定,并选择淀粉含量及支链/直链比有代表性的6个品种进行制麦试验,研究了小麦制麦芽期间淀粉含量的变化,以期探讨一种简便快捷的测定小麦及麦芽中淀粉含量的方法,并为筛选适合制麦芽小麦品种提供依据。根据碘-直链淀粉和碘-支链淀粉复合物的吸收光谱,选择直链淀粉的测定波长为631 nm和480 nm,支链淀粉的测定波长为554 nm和754 nm,依据回归方程可求出直链和支链淀粉的含量。直链淀粉的浓度在0～30μg/mL,支链淀粉在0～110μg/mL范围内符合比耳定律。制麦前后总淀粉降解程度与原小麦中支链/直链淀粉存在正相关性（P〈0.1）,相关系数r=0.802。Dual- wavelength spectrophotometry was applied to determine the amylose and amylopectin in wheat and wheat malt. The amylose and amylopectin contents of 15 wheat varieties were detemfined and 6 of the 15 varieties were selected to malt. The change of the starch content during germination was studied. The aim of the study is to find a quick method to detemrine the amylose and amylopectin of wheat and wheat malt and to offer useful data for malting. According to the absorption spectra of complexes, the I2 - amylose wavelength of 631 nm and 480 nm, and the I2 - amylopectin wavelength of 554 nm and 754 nm were selected as measuring wavelength. The content of amylose and amylopectin can be figured out from two regressive equations. Beer＇s law is obeyed in concentration of 0 ～ 30 μg/mL for amylose and 0100 μg/mL for amylopectin. The breakdown extent of starch in germination course has positive relativity with the amylopectin/amylose ratio of wheat, correlation coefficient is 0. 802 （ P 〈 0.1 ）.

关 键 词：直链淀粉 支链淀粉 双波长法 小麦 小麦芽 

分 类 号：TS211.2[轻工技术与工程—粮食、油脂及植物蛋白工程]