毛霉的产蛋白酶发酵条件优化  

Optimization of Fermentation Conditions for Proteases Produced by Mucor

作　　者：郑晓婷[1] 赵新淮[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《微生物学通报》2009年第2期193-197,共5页Microbiology China

摘　　要：从发酵豆制品中分离出1株产蛋白酶性质优良的毛霉M2,并研究M2菌株的产蛋白酶条件。研究优化得出该毛霉菌株的产酶培养基条件为:氮源为大豆分离蛋白,碳源为葡萄糖,无机盐为磷酸二氢钾、氯化钙和氯化镁;它的适宜的产酶发酵条件为:培养温度为28℃,接种量为2%,300mL三角瓶的装液量为100mL,初始pH为5,摇床转速为150r/min,培养时间为48h;发酵液蛋白酶酶活力可达4.35U/mL。凝胶电泳法测定出毛霉所分泌蛋白酶的分子量为36.4kD。A strain of Mucor named M2, which could produce protease, was isolated from a traditional fer- mented soybean product. Culture conditions of proteases produced by M2 were studied therefore. The results showed that nitrogen source and carbon source preferred for protease production were soybean protein iso- late and glucose, while inorganic salts preferred were KH2PO4, CaCl2 and MgCI2. The suitable culture con- ditions for protease production were as follows： culture temperature was 28℃, inoculation volume was 2%, liquid level was 100 mL in 300 mL triangle bottle at pH 5, rotating speed was 150 r/min and culture time was 48 h. The obtained protease activity in culture was about 4.35 U/mL. The protease produced by Mucor was analyzed with SDS-PAGE. The protease had a molecular weight of 36.4 kD.

关 键 词：毛霉 蛋白酶 发酵条件 

分 类 号：Q93[生物学—微生物学]