固相萃取-高效液相色谱法测定葡萄酒多酚物质  

Solid-phase extraction and High-performance liquid chromatography determination of polyphenols in wine

作　　者：翁鸿珍[1] 岳泰新[1] 成宇峰[1] 

机构地区：[1]包头轻工职业技术学院生物工程系,内蒙古包头014040

出　　处：《中国酿造》2009年第3期164-166,共3页China Brewing

摘　　要：通过对固相萃取柱萃取条件选择优化,确定了利用高效液相色谱法测定葡萄酒中多酚物质时最佳样品预处理条件。结果表明,乙醚作为洗脱剂时,洗脱液体积为15mL,酒样pH值为葡萄酒自然pH值时,各多酚类物质分离效果最佳,提取率均较高。The methods of solid-phase extraction were optimisated, the best conditions of sample preparation used to determrine 8 polyphenols in wine by HPLC were established. The results showed that： when the pH of wine samples was 3.2, dichloromethane volume was 15mL and ethylether as organic solvents, the 8 polyphenols in wine was separated successfully with a high extractions of 8 polyphenols.

关 键 词：固相萃取柱萃取 高效液相色谱 多酚物质 

分 类 号：O657.7[理学—分析化学]