3-脱氧葡糖松代谢酶产生菌的筛选及产酶条件  

STUDIES ON 3-DEOXYGLUCOSONE-METABOLIZING ENZYME OF FUNGI

作　　者：李湘萍[1] 莫柏立[1] 庞宗文 黄时海 梁智群 

机构地区：[1]广西大学工业测试实验中心,南宁530004

出　　处：《微生物学通报》1998年第2期71-74,共4页Microbiology China

基　　金：国家自然科学基金!29466012;广西科学基金

摘　　要：从霉菌和酵母中筛选到一株酵母（Saccharomycescerevisiae231），该菌株具有能够代谢3-脱氧葡糖松（3－deoxyglucosone）的酶，且活性较高。研究了该菌株的最适产酶条件：培养温度28℃，培养基起始pH7.0，培养时间12h，碳源、氮源分别为蔗糖、牛肉膏，添加KH_2PO_4、Ca（H_2PO_4）_2·H_2O能促进产酶。A yeast strain (saccharomyces cerevisiae 231) was selected from fungi. The stainhas 3-deoxyglucosone-metabolizing enzyme and it's activity is fairly high. The optimumconditions of enzyme production were examined. The optimum temperatUre, initial pH andcultivate bine for enzyme production were 28℃, PH7.0 and 12 hours respectively. The suitablecarbon source of the medium was sucrose and the suitable nitrogen source was beef extractEnzyme activity can increase by adding KH2PO4 or Ca(H2PO4)2. H2O.

关 键 词：美拉德反应 3-脱氧葡糖松 霉菌 酵母 

分 类 号：Q939.5[生物学—微生物学] TQ925[轻工技术与工程—发酵工程]