猪皮胶原蛋白水解产物中的抗氧化活性肽的分离及其氨基酸组分  

Separation of Antioxidative Peptides from Porcine Collagen Hydrolysis and Its Analysis of Amino Acid Composition

作　　者：朱夕波[1] 周培根[1] 李燕[1] 张艳[1] 

机构地区：[1]上海水产大学食品学院,上海200090

出　　处：《天然产物研究与开发》2009年第1期122-124,182,共4页Natural Product Research and Development

基　　金：上海市食品研究所项目基金(技04-52)

摘　　要：利用不同蛋白酶水解猪皮胶原蛋白,根据水解产物对自由基的清除率,筛选最适蛋白酶的组合,筛选抗氧化活性较高的肽类,并进行氨基酸分析。结果表明:胃蛋白酶→木瓜蛋白酶→菠萝蛋白酶为酶解胶原蛋白的最佳组合;分离纯化得到了三种具有较高抗氧化活性的肽;氨基酸组分分析显示:P1′、P2′和P3′组分具有自由基清除作用的关键氨基酸甲硫氨酸、酪氨酸和组氨酸等。The combination of the different proteases, used for hydrolyzing of the porcine collagen, and the antioxidative peptides were selected from hydrolysates based on the scavenging effect of the free radical DPPH ·,and their amino acid composition of the peptides was analyzed. The results showed that the combination of pepsin, papain and bromelain was optimum for hydrolysis of the collagen, and three kinds of the antioxidative peptides were purified. The assay of their amino acid compositions indicated that they contained the key amino acid for the antioxidation, such as Met, Lyr, His, et al.

关 键 词：胶原蛋白 水解 多肽 抗氧化活性 氨基酸 

分 类 号：R282.71[医药卫生—中药学] TS251[医药卫生—中医学]