牛乳脂肪甘油三酯中sn-2位脂肪酸组成的分析  

Study on the Constituents of Sn-2 Fatty Acid from Milk Triacylglyceride

作　　者：韩瑞丽[1] 马健 张佳程[1] 生庆海[3] 

机构地区：[1]青岛农业大学食品学院,山东青岛266109 [2]河北省乳业工程技术研究中心 [3]东北农业大学黑龙江省乳品工业技术开发中心

出　　处：《乳业科学与技术》2009年第2期71-73,共3页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

摘　　要：用胰脂酶专一水解掉牛乳脂肪甘油三酯sn-1和sn-3位置上的脂肪酸得到sn-2单甘油酯和游离脂肪酸,再通过高效液相-蒸发光散射检测法分离出sn-2位单甘油酯,甲酯化后,用GC-MS对sn-2脂肪酸组成进行分析。结果表明石家庄地区夏季牛乳脂肪sn-2位脂肪酸主要有六种,分别为:月桂酸2.57%、豆蔻酸7.68%、棕榈酸34.74%、亚油酸11.56%、油酸22.53%、硬酯酸15.21%。2-Monoacyl-sn-glycerols and free fatty acids were obtained from milk TAG upon hydrolysis of the acyl group in the 1-and 3-position of glycerol using porcine pancreatic lipase. Then 2-Monoacyl-sn-glycerols was separated by HPLC-ELSD, the 2-position fatty acid composition was determined by GC-MS of the fatty acid methyl esters. The results shows that 2-position fatty acid of milk were composed by Laurie acid 2.57%, Myristic acid 7.68%, Palmitic acid 34.74%, Linolenic acid 11.56%, Oleic acid22.53% and Stearic acid 15.21%.

关 键 词：牛乳脂肪 甘油三酯 sn-2位脂肪酸 高效液相-蒸发光散射检测器 气质联用 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]