三株沙门氏菌VBNC状态诱导条件的筛选  

Establishement of the viable but non-culturable state for three Salmonella strains with different inducing condition

作　　者：孙晓媛[1] 钱爱东[1] 李影[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118

出　　处：《中国预防兽医学报》2009年第3期193-197,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助(30471286;30700594);吉林省科技发展计划项目(20060556)

摘　　要：通过对3株沙门氏菌在不同条件下的诱导,确定沙门氏菌进入VBNC状态的条件,为进一步研究VBNC状态的发生机制奠定基础。以改变沙门氏菌培养环境为主,设计11种条件对3株沙门氏菌进行诱导培养,同时观察其可培养菌数的变化及荧光图像的变化。确定标准株S1在LB液体培养基4℃需氧培养到55d时可培养菌数降为零,同时菌体变为球杆菌,可认为其进入VBNC状态。To study the mechanism of the viable but non-culturable （VBNC） state, the conditions for inducing VBNC state of three Salmonella strains were established. Salmonella were cultured under different culture conditions and the bacteria numbers and the fluorescence image change was monitored. The S1 strain was considered entering the VBNC state when culturable number reduced to zero and the mycelium becomes the coccobacillus after cultured in the LB liquid media at 4 ℃ in the presence of oxygen for 55 days.

关 键 词：沙门氏菌 VBNC 诱导 筛选 

分 类 号：S852.613[农业科学—基础兽医学]