一级反应网络法求单底物酶促反应的米氏常数  

Determination of the Michaelis Constant of Single Substrate Enzyme catalysed Reaction by the First order Reaction Network Theory

作　　者：王静[1] 王刚[1] 刘武钧 魏泽英[1] 谭辉玲[1] 

机构地区：[1]重庆大学化学化工学院

出　　处：《重庆大学学报（自然科学版）》1998年第2期125-130,共6页Journal of Chongqing University

摘　　要：以可溶性淀粉为底物，研究了糖化酶（ＥＣ３．２．１．３）的动力学规律。淀粉水解反应具有单底物酶促反应的特征，且当［ｓ］Ｋｍ时，服从拟一级反应规律。根据一级反应网络理论，采用单纯形加速法拟合酶促淀粉水解反应的过程曲线求各速率常数和米氏常数。The kinetics of free glucoamylase (E C 3.2.1.3) was studied with soluble starch as substrate.The hydrolysis reaction of soluble starch has the characteristics of single substrate enzyme catalysed reaction and when K m,it obeys the law of apparent first order reaction.According to the first order reaction network theory,the time course of the starch hydrolysis and the glucose production have been dealt with and the apparent rate constants and michaelis constant are obtained by making use of the simple acceleration method.

关 键 词：米氏常数 糖化酶 一级反应网络法 淀粉 水解 

分 类 号：Q556.2[生物学—生物化学]