回归正交法优化杜仲叶中总黄酮提取工艺的研究  

Study on optimizing extraction technology of total flavonoids in Eucommia ulmoides olive leaves through orthogonal regression design

作　　者：杨海涛[1] 刘军海[1] 

机构地区：[1]陕西理工学院化学与环境科学学院,陕西汉中72300

出　　处：《食品工业科技》2009年第3期248-250,共3页Science and Technology of Food Industry

基　　金：陕西理工学院教改项目(XJG0717)。

摘　　要：以杜仲叶为原料,采用索氏提取法对其有效成分总黄酮进行提取。以正交实验考察了提取过程中的各影响因素,再以回归正交实验优化了提取工艺。结果表明:影响杜仲叶总黄酮提取率的因素顺序为:乙醇浓度>料液比>提取时间>提取次数>提取温度>pH;最佳工艺条件为:提取时间38.66min、料液比1∶12.09、乙醇浓度67.14%,在最佳工艺条件下提取率可达1.55%。The total flavonoids in Eucommia ulmoides olive leaves were extracted by soxhlet extraction method.The influence factors were studied through orthogonal experiment,and then the extraction technologys were optimized through orthogonal regression design.The results showed that：the factors which affected the extraction rate were ethanol concentration 〉 ratio of material to solvent 〉 extraction time 〉 extraction times 〉 extraction temperature 〉 pH.The optimum conditions were ： extraction time was 38.66min, ratio of material to solvent was 1：12.09, and ethanol concentration was 67.14% ,the extraction could up to 1.55% under the optimum conditions.

关 键 词：杜仲叶 总黄酮 提取 回归正交实验 

分 类 号：TS201.2[轻工技术与工程—食品科学]