粪鬼伞菌丝体提取液的抗菌活性  

Study on the Antibacterial Activity of Extracts from the Mycelium of Submerged-Cultured Coprinus sterqulinus Fr.

作　　者：王磊[1] 邱芳萍[1] 王志兵[1] 解耸林[1] 田文莹[1] 

机构地区：[1]长春工业大学生物工程学院,吉林长春130012

出　　处：《食品与生物技术学报》2009年第2期272-275,共4页Journal of Food Science and Biotechnology

基　　金：吉林省教育厅科技计划项目(200660)

摘　　要：以深层发酵得到的粪鬼伞菌丝体为原料,分别用水和80%的乙醇溶液进行超声波浸提,对浸提液进行体外抗菌实验,然后用与总糖浓度相同的多糖溶液和水提液对比进行抗菌试验。结果表明,水提液对大肠杆菌和枯草芽孢杆菌的最低抑菌浓度(MIC)为3.2 mg/mL,对金黄色葡萄球菌的MIC为1.6 mg/mL;对大肠杆菌的最低杀菌浓度(MBC)为12.5 mg/mL,对金黄色葡萄球菌和枯草芽孢杆菌的MBC为25 mg/mL;对青霉和黑曲霉没有作用;醇提液对大肠杆菌和枯草芽孢杆菌的MIC为50 mg/mL,对金黄色葡萄球菌、青霉和黑曲霉没有作用;总糖质量浓度相同的多糖溶液和水提液具有等效的抑菌和杀菌能力。因此,粪鬼伞多糖就是水提液中的主要抗菌活性物质。The antibacterial effects of the extracts from the submerged-cultured mycelium of Coprinus sterquilinus Fr were studied. The extracts were prepared in water and 80% ethanol respectively. 10.01 mg/mL polysaccharide solvent, which had the same concentration of sugar with the 5% water extract, both of them were tested their antibacterial activity. The water extracts of Coprinus sterquilinus Fr. mycelium showed high antibacterial effect than that of ethanol extracts. The MIC was 3.2 mg/mL for E. coli and B. subtilis, 1.6 mg/mL for S. aureus. The MICethanol exlract was 50 mg/mL for E. coli and B. subtilis. The MBC was 25 mg/mL for B. subtilis and S. aureus, 12.5 mg/mL for E. coli respectively. The water and ethanol extracts had no significant antibacterial effect on fungi. Polysaccharide solvent and water extract of the same concentration had the similar bactericidal capability. The results present here demonstrated that polysaccharide was the main antibacterial active substance of the water extract from the Coprinus sterquilinus Fr. mycelium.

关 键 词：粪鬼伞 菌丝体 提取液 抗菌活性 多糖 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]