In Vitro Cytotoxicity of Polyphosphoester as a Novel Injectable Alveolar Replacement Material  

作　　者：张智星 毛靖 冯祥礼 肖建中 邱进俊 

机构地区：[1]Department of Orthodontics,Tongji Hospital,Tongji Medical College, Huazhong University of Science and Technology [2]Department of Nursing, Tongji Medical College, Huazhong University of Science and Technology [3]College of Materials Science and Engineering,Huazhong University of Science and Technology [4]College of Chemistry, Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2008年第5期604-607,共4页华中科技大学学报（医学英德文版）

基　　金：a grant from the National High Technology Research and Development Program of China (863 Program, No. 2006AA03Z0443)

摘　　要：The aim of this study was to investigate the in vitro cytotoxicity of polyphosphoester polymer used as a novel injectable alveolar bone substitutes for controlled delivery of tetracycline. Cell culture medium was exposed to the polymer （0.01-10 mg/mL） for 24 h. The L-929 mouse fibro- blasts were then exposed to the treated cell culture medium for 24 h. Finally, cell viability and growth were assessed by using MTT assay and Alamar Blue assay. No significant cytotoxicity of the polyphosphoester against L-929 mouse fibroblasts was observed at a concentration up to 10 mg/mL （P〉0.05）. The two evaluation methods showed no significant differences （P〉0.05）. This study suggests that polyphosphoester does not demonstrate any significant toxic effects to cells in vitro and has the potential to be used both as a medical device and as scaffolds in tissue engineering applications.The aim of this study was to investigate the in vitro cytotoxicity of polyphosphoester polymer used as a novel injectable alveolar bone substitutes for controlled delivery of tetracycline. Cell culture medium was exposed to the polymer （0.01-10 mg/mL） for 24 h. The L-929 mouse fibro- blasts were then exposed to the treated cell culture medium for 24 h. Finally, cell viability and growth were assessed by using MTT assay and Alamar Blue assay. No significant cytotoxicity of the polyphosphoester against L-929 mouse fibroblasts was observed at a concentration up to 10 mg/mL （P〉0.05）. The two evaluation methods showed no significant differences （P〉0.05）. This study suggests that polyphosphoester does not demonstrate any significant toxic effects to cells in vitro and has the potential to be used both as a medical device and as scaffolds in tissue engineering applications.

关 键 词：POLYPHOSPHOESTER CYTOTOXICITY MTT assay Alamar Blue test 

分 类 号：R783.1[医药卫生—口腔医学]