ε-聚赖氨酸的生物合成与降解及其应用研究进展  

Advance in Research on ε-Polylysine Biosynthesis and Its Degradation and Application

作　　者：史应武[1,2] 娄恺[2] 李春[1] 

机构地区：[1]石河子大学农学院/新疆兵团化工绿色过程重点实验室,新疆石河子832003 [2]新疆农业科学院微生物应用研究所,乌鲁木齐830091

出　　处：《中国农业科学》2009年第3期1009-1015,共7页Scientia Agricultura Sinica

基　　金：农业部“948”项目(2006-G62);国家科技基础条件平台项目(2005DKA21201-12)

摘　　要：ε-聚赖氨酸(ε-PL)是由赖氨酸的α-氨基与ε-羧基通过肽键结合成的同型聚合物,在高温与酸碱环境中很稳定,作为天然食品防腐剂已经进入工业化发酵生产阶段。ε-PL产生菌通常在细胞膜上具有ε-PL降解酶,以达到保护自己的目的,但ε-PL的微生物合成机理尚不清楚。本文从ε-PL的理化性质、抑菌活性、菌株分离筛选、生物合成与分子遗传学、发酵工艺优化、分离纯化及应用等方面综述了ε-PL的最新研究进展与动态。ε-polylysine （ε-PL） is a home-poly-amino acid characterized by the peptide bond between the carboxyl and ε-amino groups of L-lysine, which is stable at high temperature and under both acidic and alkaline conditions. Due to this antimicrobial activity, ε-PL is now industrially produced as a natural antiseptic agont by a fermentation process, ε-PL producers commonly possess membrane-bound ε-PL-degrading aminopeptidase, which might play a role in self-protection. It is unknow about the biosynthetic mechanism of ε-PL. This review focuses on the physicochemical properties of ε-polylysine, antimicrobial activities of ε-polylysine, isolation and screening of producing strains of ε-polylysine, biosynthesis and molecular genetics of ε-polylysine, fermentation process optimization of ε-polylysine, purification and application of ε-polylysine.

关 键 词：Ε-聚赖氨酸 生物合成 链霉菌 天然防腐剂 

分 类 号：TS202.3[轻工技术与工程—食品科学]