Matrix Interference in Serum Total Thyroxin (T_4) Time-resolved Fluorescence Immunoassay (TRFIA) and Its Elimination With the Use of Streptavidin-biotin Separation Technique  

作　　者：WU Feng-bo, HE You-feng, HAN Shi-quan 

出　　处：《Annual Report of China Institute of Atomic Energy》2001年第0期100-101,共2页中国原子能科学研究院年报（英文版）

摘　　要：In development of serum total thyroxin TRFIA using the surface second-antibody (S-Ab) as separation agent, a significant bias of measurement caused by matrix interference when the surface S-Ab shows a relatively low binding capacity for primary anti-T4 monoclonal antibody (McAb) is studied. The bias ranges from 10% to 78%, depending on the matrix of individual samples and the binding capacity of the surface S-Ab prepared. So, a new separation system based on the use of a highly active surface streptavidin and biotinylated anti-T4 McAb is employed. The results

关 键 词：TRFIA BIOTIN SERUM STREPTAVIDIN MONOCLONAL Fluorescence resolved coated maintained precise 

分 类 号：R446.61[医药卫生—诊断学]