Cloning and expression of Kluyveromyces fragilis LAC4 gene  

作　　者：霍克克 李育阳 

机构地区：[1]Institute of Genetics,Fudan University,Shanghai 200433,China

出　　处：《Science China Chemistry》1995年第11期1332-1340,共9页中国科学（化学英文版）

摘　　要：The genomic library of Kluyveromyces fragilis was constructed in E.coli TG1,and 5β-galactosidase gene(LAC4)clones have been obtained from the library by complementation of theKluyveromyces lactis lac4-8 mutation.The studies on the structure and the function of the LAC4 gene revealedthat(i)the gene can also complement E.coli lacZ mutation;(ii)the physical map of the K.fragilis LAC4gene was very similar to that of K.lactis;(iii)the β-galactosidase levels expressed by the clone strains weremuch higher than that expressed by the original strain;(iv)the variation of the β-galactosidase level of differ-ent clone strains induced by lactose or galactose was related to the retained degree of the 5’ flanking region ofLAC4 gene,suggesting that there migth he a lactose specific transcription activating element in the region.The genomic library of Kluyveromyces fragilis was constructed in E.coli TG1,and 5 β-galactosidase gene(LAC4)clones have been obtained from the library by complementation of the Kluyveromyces lactis lac4-8 mutation.The studies on the structure and the function of the LAC4 gene revealed that(i)the gene can also complement E.coli lacZ mutation;(ii)the physical map of the K.fragilis LAC4 gene was very similar to that of K.lactis;(iii)the β-galactosidase levels expressed by the clone strains were much higher than that expressed by the original strain;(iv)the variation of the β-galactosidase level of differ- ent clone strains induced by lactose or galactose was related to the retained degree of the 5' flanking region of LAC4 gene,suggesting that there migth he a lactose specific transcription activating element in the region.

关 键 词：KLUYVEROMYCES fragilis GENOMIC LIBRARY Β-GALACTOSIDASE GENE CLONING expression 

分 类 号：Q78[生物学—分子生物学]