生物柴油副产物甘油中磷脂酰胆碱的提取纯化研究  

Study on purification of phosphatidylcholine from crude glycerol byproduct of biodiesel

作　　者：张雯[1] 石云慧[1] 王承明[1] 

机构地区：[1]华中农业大学生物质能研发中心,华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品工业科技》2009年第4期203-207,共5页Science and Technology of Food Industry

基　　金：国家"十五"重大科技专项(2001BA501A20);湖北省重大科技攻关项目(2006AA201B32)

摘　　要：以生物柴油副产物甘油中分离得到的粗磷脂为原料,采用乙醇提取、减压蒸馏浓缩制备较高纯度磷脂酰胆碱(PC)。通过响应曲面设计考察了料液比、提取温度、提取时间、搅拌速度对提取率的影响。优化的最佳提取条件为:液料比42:1(mL/g),提取温度46℃,提取时间38min,搅拌速度328r/min,提取一次。经实验验证,提取率达到78.2%,PC含量由原来的46%提高到77%。Purification of phosphatidylcholine （PC）from the phospholipids which extracted from the glycerol byproduct of biodiesel was studied by usage of ethanol. The effects of the materials fluids ration, extraction temperature,extraction time and agitating rate on extraction of PC were been described.The optimized extraction conditions was obtained by the response surface methodology, and the fluids materials ratio was 42 ： 1 （ mL/g）, extraction temperature was 46℃, extraction time was 38min, and agitating rate was 328r/min. The optimized extraction rate was 78.2% ,the PC purity was up to 77% from 46%.

关 键 词：生物柴油 甘油 提纯 磷脂酰胆碱 响应曲面 

分 类 号：TS201.1[轻工技术与工程—食品科学]