营养强化剂乙二胺四乙酸铁钠中氨基三乙酸的检测方法研究  

Determination of nitrilotriacetic acid in sodium iron(Ⅲ)ethylenediaminetetraacetate for nutritional fortifier

作　　者：魏峰[1] 霍军生[2] 于波[2] 孙静[2] 黄建[2] 狄蕊[1] 

机构地区：[1]黄山学院化学系,安徽黄山245041 [2]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《食品工业科技》2009年第4期321-323,共3页Science and Technology of Food Industry

摘　　要：建立了用反相离子对液相色谱分离和测定营养强化剂乙二胺四乙酸铁钠(NaFeEDTA)中氨基三乙酸(NTA)的方法。该方法将样品与二价铜盐进行络合后,以ZorbaxC8(4.6mm×150mm,5μm)色谱柱作为固定相,含10%(v/v)甲醇、0.2%四丁基氢氧化铵(TBAOH)和pH为7.3的磷酸水溶液作为流动相,流速为2.00mL/min进行分离,在紫外波长244nm下检测。NaFeEDTA中NTA检测的平均回收率为97.5%,最低检出限为20mg/kg。本方法灵敏、准确,可以用于NaFeEDTA中NTA的检测。A reversed-phase ion-pair high performance liquid chromatographic method was developed for determination of nitrilotriacetic acid（NTA） in sodium iron（Ⅲ） ethylenediaminetetraacetate（NaFeEDTA） for nutritional fortifier.After chelation with cupric ion,the sample could be separated on a Zorbax C8 column （4.6mm × 150mm, 5μm）.The mobile phase was phosphoric acid solution containing 10% （v/v）methanol and 0.2% tetrabutyl ammonium hydroxide（ pH = 7.3）, at a flowrate of 2.00mL/min. NTA was detected at 244nm.The average recovery was 97.5% ,the detection limit of NTA in NaFeEDTA was 20mg/kg.This sensitive and accurate method could be used in determination of NTA in NaFeEDTA.

关 键 词：氨基三乙酸 乙二胺四乙酸铁钠 反相离子对高效液相色谱 

分 类 号：TS207[轻工技术与工程—食品科学]