Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats  

作　　者：Hai-Qiang Zhang Tomas T Ding Jun-Sheng Zhao Xin Yang Hai-Xia Zhang Juan-Juan Zhang Yun-Long Cui 

机构地区：[1]Qingdao Research Center of Microecology-Engineering Technology, Jiaonan 266400, Shandong Province, China [2]BRITE/Department of Pharmaceutical Sciences, North Carolina Central University, Durham, NC27707, United States [3]Pathology Department of People's Hospital,Jiaonan 266400, Shandong Province, China [4]Qingdao East Sea Pharmaceutical Co. Ltd,Jiaonan 266400, Shandong Province, China

出　　处：《World Journal of Gastroenterology》2009年第15期1821-1828,共8页世界胃肠病学杂志（英文版）

基　　金：The Model Subject of High-Tech Industrializing Conversion of Series Microecological New Drugs,National Development and Reform Commission High-Tech,[2004]2078

摘　　要：AIM： To evaluate the therapeutic effects of a probiotic supplement （Clostridium butyricum, CGMCC0313 ） in a chemically-induced rat model of experimental colitis. METHODS： An experimental ulcerative colitis model was established by rectal injection of oxazolone into the colon of 40 Wistar rats randomly divided into four groups. The positive control group was sacrificed 3 d after colitis onset. The remaining groups were fed daily with either 2 mL of C. butyricum （2.3 × 10^11 CFU/L）, 2 mL of mesalamine （100 g/L）, or 1 mL of sodium butyrate （50 mmol/L） for 21 d. The animals＇ body weight, behavior, and bowel movements were recorded weekly. After sacrifice, visual and microscopic observations of pathological changes of colon tissue were made, body weight and wet colon mass index were measured and recorded, and serum levels of interleukin-23 （IL-23） and TNF-α were measured using ELISA. Expression of calcitonin gene-related peptide in colon tissue was measured by RT-PCR. Finally, changes in rat intestinal microflora status were measured in all groups.lowered the serum levels of both IL-23 and tumor necrosis factor-α （TNF-α） with similar or even better efficiency than that of mesalamine or sodium butyrate. The rat intestinal flora appeared to recover more quickly in the group treated with C. butyricum than in the mesalamine and sodium butyrate groups. Finally, we found that the expression level of calcitonin gene related peptide was elevated in colon tissue in the sodium butyrate treated group but not in the C. butyricum or mesalamine treated groups, indicating a sensitization of colon following sodium butyrate treatment. CONCLUSION： In our experimental colitis model, treatment with C. butyricum CGMCC0313, a probiotic supplement, is at least as efficient as treatment with mesalamine.AIM:To evaluate the therapeutic effects of a probiotic supplement(Clostridium butyricum,CGMCC0313)in a chemically-induced rat model of experimental colitis. METHODS:An experimental ulcerative colitis model was established by rectal injection of oxazolone into the colon of 40 Wistar rats randomly divided into four groups.The positive control group was sacrificed 3 d after colitis onset.The remaining groups were fed daily with either 2 mL of C.butyricum(2.3×1011 CFU/L), 2 mL of mesalamine(100 g/L),or 1 mL of sodium butyrate(50 mmol/L)for 21 d.The animals’body weight, behavior,and bowel movements were recorded weekly. After sacrifice,visual and microscopic observations of pathological changes of colon tissue were made,body weight and wet colon mass index were measured and recorded,and serum levels of interleukin-23(IL-23) and TNF-αwere measured using ELISA.Expression of calcitonin gene-related peptide in colon tissue was measured by RT-PCR.Finally,changes in rat intestinal microflora status were measured in all groups. RESULTS:We found that treatment with C.butyricum lowered the serum levels of both IL-23 and tumor necrosis factor-α(TNF-α)with similar or even better efficiency than that of mesalamine or sodium butyrate. The rat intestinal flora appeared to recover more quickly in the group treated with C.butyricum than in the mesalamine and sodium butyrate groups.Finally,we found that the expression level of calcitonin gene related peptide was elevated in colon tissue in the sodium butyrate treated group but not in the C.butyricum or mesalamine treated groups,indicating a sensitization of colon following sodium butyrate treatment.CONCLUSION:In our experimental colitis model, treatment with C.butyricum CGMCC0313,a probiotic supplement,is at least as efficient as treatment with mesalamine.

关 键 词：Clostridium butyricum INTERLEUKIN-23 Tumor necrosis factor-m Calcitonin gene relatedpeptide COLITIS 

分 类 号：R574.62[医药卫生—消化系统] R975[医药卫生—内科学]