高效液相色谱法测定必特螺旋霉素发酵液中有机酸  

Determination of organic acids in bitespiramycin fermentation broth by high performance liquid chromatography

作　　者：刘健[1] 王永红[1] 庄英萍[1] 付启伟[1] 储炬[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《工业微生物》2009年第2期59-62,共4页Industrial Microbiology

基　　金：上海市重点学科建设项目(B505);上海市科委科技攻关项目(064319033)

摘　　要：必特螺旋霉素是运用基因工程技术获得的工程菌产生的一组以异戊酰螺旋霉素为主要成分的多组分新型抗生素,其前体为乙酸、丙酸、丁酸和异戊酸等有机酸。本研究利用高效液相色谱法以0.01 mol/L磷酸缓冲液(pH2.3)和甲醇为流动相,分别在反相C_8(α-酮戊二酸、乙酸、柠檬酸、琥珀酸、丙酸)和C_(18)(丁酸、异戊酸)柱上定量测定了必特螺旋霉素前体酸和三羧酸循环相关有机酸。所建立的测定方法的相对标准偏差为0.10%～0.42%,回收率为93.19%～102.08%。Bitespiramycin, mainly 4＇ -isovaleryl-spiramycins, a novel antibiotic of sixteen-member macrolide derived from spiramycin, was produced by a genetic engineering microbial strain. Organic acids take profound effect on the biosynthesis of bitespiramycin. In this research, a method for determining the organic acids in bitespiramycin fermentation broth by high performance liquid chromatography based on C8 and C18 column and using 0.01 mol/L phosphoric acid buffer solution （pH 2.3） and methanol as mobile was investigated. The relative standard deviations were 0.10% --0.42%, and the recovery ratios were 93.19 % -- 102.08 %.

关 键 词：高效液相色谱法 有机酸 必特螺旋霉素 发酵液 

分 类 号：TQ927[轻工技术与工程—发酵工程] O657.72[理学—分析化学]