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作 者:陆胜民[1,2] 袁晓阳[3] 杨颖[1] 夏其乐[1] 邢建荣[1] 郁志芳[3]
机构地区:[1]浙江省农业科学院食品加工研究所,浙江杭州310021 [2]浙江万里学院食品科学系,浙江宁波315100 [3]南京农业大学食品学院,江苏南京210095
出 处:《浙江农业学报》2009年第2期101-105,共5页Acta Agriculturae Zhejiangensis
基 金:宁波市科研项目(2006C100072)
摘 要:进行了人工发酵生产臭冬瓜的工艺研究,通过对20种菌种组合方式的试验,最终得到两种可以产生理想气味且气味能够保持的组合,分别为:(1)短小芽孢杆菌、腐败希瓦菌、异常汉逊酵母分别按2%(V/V)的接种量接种;(2)短小芽孢杆菌、腐败希瓦菌、极小棒杆菌分别按2%的接种量接种并发酵3d后,再分别接入2%(V/V)的异常汉逊酵母、酿酒酵母和植物乳杆菌。通过正交试验选取的最佳发酵工艺条件为:短小芽孢杆菌、腐败希瓦菌、异常汉逊酵母各自按照2%(V/V)的接种量接种于含2%(V/V)豆豉汁和1%(W/V)氯化钠的液体冬瓜发酵基中,30℃发酵10d后,在发酵体系中投入能够被发酵液覆盖量以下的冬瓜块,继续30℃发酵10~15d。Technology of producing wax gourd with strong odor by inoculated fermentation was studied in this paper. Among 20 combinations of isolated strains, two of them were ideal in flavor formations: (1) 2% (V/V) Bacillus pumilus, 2% (V/V) Shewanellaputrefaciens, 2% (V/V) Hansenula anomala were inoculated; (2) 2% (V/V) B. pumilus, 2% (V/V) S. putrefaciens, 2% (V/V) Corynebacterium minutissimum were inoculated, and then 2% (V/V) Hansenula anomala, 2% (V/V) Saccharomyces cerevisiae and Lactobacillus plantarum were inoculated three days after the first inoculation. Through orthogonal tests, the panel organoleptic evaluation indicated that optimum mode of inoculated strains was that 2% (V/V) B. p umilus, 2% (V/V) S. putrefaciens, 2% (V/V) H. anomala were inoculated in wax gourd juice including 2% (V/V) lobster sauce juice and 1% (W/V) salt. The inoculated liquids were fermented for 10 days at 30℃, then the wax gourd was put in to continue the fermentation for another 10- 15 days at 30℃.
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