美味牛肝菌多糖发酵工艺的研究  

Optimization of Culture Parameters Affecting Polysaccharide Production by a Strain of Boletus edulis Grown in Submerged Culture

作　　者：阳飞[1] 张华山[1] 董殿波[1] 顾亚婧[1] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068

出　　处：《食用菌学报》2009年第1期67-72,共6页Acta Edulis Fungi

摘　　要：本文对美味牛肝菌（Boletus edulis）液体发酵生产粗多糖的培养基和发酵条件进行了筛选。在实验范围内，美味牛肝菌发酵生产粗多糖的最适培养基配方为：马铃薯10.0％，蔗糖2.0％，蛋白胨0.6％，KH2PO4 0.3％，MgSO4·7H2O 0.2％，CaCO3 30.2％，ZnSO4·7H2O 0.1％。在初始pH5.0-5.4。振荡转速120r／min，培养温度25-28℃，250mL三角瓶中装液量40％，加10颗玻璃珠，发酵时间5d时摇瓶培养粗多糖产量最高，达3.161g／L。经10L机械搅拌发酵罐放大动态实验，研究pH值、溶氧、菌丝生物量（湿重）以及粗多糖产量的变化，可知最适发酵时间为96h。The effects of selected culture parameters on polysaccharide production by a strain of Boletus edulis are described. Optimal conditions for small-scale polysaccharide production in submerged flask cultures were as follows： a fermentation medium consisting of 10% potato, 2% sucrose, 0.6% peptone, 0.10% ZnSO4·7H20, 0.2% CaCO3, 0.3% KH2PO4 and 0.2% MgSO4·7H2O, pH5.0-5.4, 120r/min shaking speed, temperature 25-28℃, 40% medium loading ratio, 10 glass beads per flask, and a 5-day incubation period.Under these conditions, polysaccharide production reached a peak of 3.161 g/L. In 10-L fermenter cultures using the same medium, pH, temperature, a 50% medium loading ratio, a similar stirrer speed, and an aeration rate of 0.8vvm, highest polysaccharide yields were obtained after four days.

关 键 词：美味牛肝菌 发酵条件 摇瓶培养 发酵罐 

分 类 号：S646[农业科学—蔬菜学]