青梅酒中苯甲醛含量的测定  

Determination of Benzaldehyde Content in Plum Fruit Wine by High Performance Liquid Chromatography(HPLC)

作　　者：高敏[1] 曾新安[1] 肖利民[2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]长沙理工大学化学与生物工程学院,湖南长沙410076

出　　处：《酿酒科技》2009年第5期110-112,共3页Liquor-Making Science & Technology

基　　金：广东省糖品绿色加工国际研发中心(2006A50105002;2008B050400002);广东省科技计划项目"安全加工中心"(2007B080401010);广东省教育部产学研结合项目(2007A090302053)资助

摘　　要：建立了高效液相色谱法测定青梅酒中苯甲醛的方法。色谱条件为色谱柱:AtlantisT3柱(150mm×4.6mm,5.0μm);流动相:乙腈-水(1:1);流速:0.5mL/min;柱温:25℃;紫外检测器的检测波长249nm。当苯甲醛的浓度在1～100mg/L范围时,色谱峰面积与苯甲醛浓度呈线性相关,相关系数r2=0.9999,最低检出限为0.1mg/L。该方法简便快速,结果准确可靠,为青梅酒中苯甲醛含量的测定提供了一种高效快速的测定方法。研究还表明青梅酒氧化后苯甲醛含量明显降低,可以作为评价青梅酒氧化程度的一个重要指标。A method （HPLC） for the determination ofbenzaldehyde content in green plum fruit wine had been developed.The technical parameters were summed up as follows： Atlantis T3 column （ 150 mm×4.6 mm, 5.0 μm）and ultraviolet light （UVL） detector were used, the detected wavelength was set at 249mn, and acetonitrile-water （1：1） was used as mobile phase, the correlation coefficient was 0.9999 when benzaldehyde content was within the range of 1 - 100 mg/L and the minimum detection limit was 0.1 mg/L. Such method was proved to be simple, rapid and accurate. The results also suggested that the content ofbenzaldehyde in green plum fruit wine dropped evidently after the oxidation （which could be used as an important index to evaluate the oxidation degree of plum fruit wine）.

关 键 词：分析检测 青梅酒 苯甲醛 高效液相色谱法 

分 类 号：TS261.7[轻工技术与工程—发酵工程]