胎儿弯杆菌表面蛋白SapA抗原单克隆抗体的制备  

Preparation of monoclonal antibodies against the surface layer protein SapA antigen of Campylobacter fetus

作　　者：赵海玲[1,2] 林靖凯[2] 杜艳芬[2] 刘慧芳[2] 司微[2] 王春来[2] 王聃[2] 赫明雷[2] 彭玮[2] 杨金国[2] 刘思国[2] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室动物细菌病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2009年第5期409-411,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家“十一五”科技支撑计划项目(2007BAD40B01)

摘　　要：用原核表达的重组胎儿弯杆菌毒力因子表面蛋白(rSapA-N)免疫小鼠,采用细胞融合技术,共获得2株抗rSapA-N的单克隆抗体(MAb),经Ig亚类鉴定,均为IgG1,轻链为κ链;Western blot证实这2株MAb均能与rSapA-N发生特异性反应,而与其它蛋白则不发生反应;以这2株MAb的杂交瘤细胞制备腹水,效价达到1∶100 000和1∶60 000。本研究制备的MAb,为研究和检测胎儿弯杆菌提供了一种重要的手段。To produce monoclonal antibody （MAb） against surface layer proteins （rSapA-N） of Campylobacter fetus, BALB/c mice were immunized with the recombinant plasmid pET32a-SapA-N. Spleen cells were collected and fused with SP2/0 cells. Two hybridoma cell clones secreting antibody against rSapA-N were obtained. Both MAbs belong to IgG1 subclass with K chain; Western blot analysis showed that both MAbs reacted with recombinant rSapA-N protein; The MAbs developed in this study could serve as an important tool in the research of the Campylobacter fetus.

关 键 词：胎儿弯杆菌表面蛋白SapA 免疫 单克隆抗体 

分 类 号：S852.612[农业科学—基础兽医学]