织纹螺的毒素成分分析  

Component analysis of toxins in Nassarius

作　　者：苏捷[1] 张农[1] 刘海新[1] 李庐峰[1] 

机构地区：[1]福建省水产研究所,福建厦门361012

出　　处：《上海海洋大学学报》2009年第3期376-380,共5页Journal of Shanghai Ocean University

基　　金：福建省科技厅资助项目(2004I010)

摘　　要：近年来,我国常有食用织纹螺中毒事件发生,由于织纹螺在世界各地海区分布广泛,种类繁多,所含的毒素成分也不完全相同,目前对织纹螺的毒素组成还存在较大的分歧。国内学者认为织纹螺毒素主要成分是麻痹性贝毒(PSP),认为织纹螺毒素可能是通过食用赤潮中有毒的藻类在体内蓄积产生。通过动物实验、液相色谱方法对福建沿海的有毒织纹螺的毒素进行比较分析,发现织纹螺的毒素中的主要成分是河豚毒素(TTX)。再用液质联用的方法对织纹螺的毒素成分作进一步分析研究,发现液质联用的条件可以很好地分离和确定样品中的TTX及其衍生物。实验研究表明织纹螺的毒素主要成分是TTX,同时还含有TTX的4位空间异构体(4-ep iTTX)、脱水河豚毒素(anhydroTTX)、脱氧河豚毒素(deoxyTTX)、单加氧河豚毒素(11-oxoTTX)等TTX的衍生物。Poisoning incidents caused by eating Nassarius have been reported frequently in the last several years in China. Various Nassarius are widespread distributed in sea areas of the world and toxins they have are also different, so there are many disputations in what toxins Nassarius are made up. Some experts believe that the toxin contained in Nassarius is paralytic shellfish poisoning which may be eaten through deleterious algae during red tide. The toxins of Nassarius had been analyzed by animal experiment and high performance liquid chromatography （HPLC）. We found the main component of Nassarius toxins is tetrodotoxin （TXX）. Furthermore, the high-performance liquid chromatography-mass spectrometry （LC-MS） was used in this paper, and the ramification such as 4-epiTTX, anhydroTTX, deoxy TTX, and 11-oxoTTX also had been found in Nassarius.

关 键 词：织纹螺 液相色谱 液质联用 河豚毒素 

分 类 号：R996.3[医药卫生—毒理学]