Down-regulation of APLP1 mRNA expression in hippocampus of pilocarpine-induced epileptic rats  

APLP1基因在皮罗卡平致痫大鼠海马组织中表达水平降低(英文)

作　　者：王成[1] 游自立[1] 张丁丁[2] 

机构地区：[1]电子科技大学生命科学与技术学院,神经信息教育部重点实验室 [2]四川省医学科学院,四川省人民医院分子遗传研究中心

出　　处：《Neuroscience Bulletin》2009年第3期109-114,共6页神经科学通报（英文版）

基　　金：supported by the National Basic Research Development Program of China (No.2006CB504501);National Key Laboratory of Modern Communication Foundation of China (9140C1101050701);the National Natural Sciences Foundation of China (No.30525030)

摘　　要：Objective To investigate the expression of amyloid beta precursor-like protein 1 （APLP 1） gene on the transcription level in hippocampus of pilocarpine-induced epileptic rats. Methods Epileptic rats were developed by LiCl （3 mmol/kg, i.p.） approximately 20 h prior to pilocarpine （30 mg/kg, i.p.） administration. The 3＇ end partial sequence of rat APLP1 gene was cloned, and the expression levels of APLP1 mRNA in hippocampus of epileptic rats at 6 h, 30 h, 7 d and 15 d were determined by semi-quantitative RT-PCR. Results The 3＇ end partial sequence of rat APLP1 gene shared a 97% homology with that of mice, and 90% with that of human. The APLP1 amino acid sequence of rat was identical with that of mouse, but was different from that of human in 3 residues. Moreover, pilocarpine induced a significant down-regulation ofAPLP1 mRNA expression at 6 h after epilepsy initiation （P 〈 0.05）, and at 30 h, this down-regulation became more dramatic （P 〈 0.01）, which lasted till day 15 （P 〈 0.01）. Conclusion The 3＇ end ofAPLP1 gene is highly conserved, and APLP1 mRNA expression is kept at low level in hippocampus of pilocarpine-induced epileptic rats.目的本实验以皮罗卡平(Pilocarpine)致痫大鼠作为研究模型,探讨了致痫后大鼠海马APLP1mRNA表达的变化。方法经腹腔注射氯化锂(3mmol/kg)加皮罗卡平(30mg/kg)后,大鼠出现癫痫持续状态。将大鼠APLP1基因3'端进行克隆,并利用半定量RT-PCR检测了致痫后6h,30h,7d,15d时大鼠海马APLP1基因mRNA表达的变化。结果大鼠APLP1基因3'端与小鼠和人类APLP1基因的同源性分别为97%和90%,其氨基酸序列与小鼠相同,但与人的APLP1有三个碱基存在差异。在致痫6h后,海马APLP1mRNA表达水平显著下降(P<0.05),并在致痫后30h呈极低表达(P<0.01),一直持续到致痫后15d。结论APLP1基因3'端高度保守,APLP1基因mRNA的表达持续保持低水平。

关 键 词：EPILEPSY APLP1 RT-PCR HIPPOCAMPUS RAT 

分 类 号：Q3[生物学—遗传学]